Comments from ComPAIR
1) Very concise and well written! Could perhaps include a comment on the scenario that you would not get the expected result? How could that be exlained? Is it possible they are still involved and needs additional experiments or can you exclude them and go look for other proteins instead?
2) I thought this method of looking at invertebrates and extrapolating that to vertebrates in really interesting and something I haven’t thought much about before. I think the background was well researched but the other parts need a bit more work.
Which system are you doing the experiment in (fly)? If so I think it could be stated more directly. Overall, I think the idea was really good but all the parts needs to be edited for clarity.
3) Your introduction was well-written and had a good flow to it. Also, you had a great setup for the experimental plan so far.
I am confused on whether the “in this knockout screen” was supposed to be potential results? If so, you may want to expand more on them.
Overall, I would say it looks good thus far 🙂
4) Your paper was very thorough on the introduction and it sounds really interesting! Based on feedback I received from doing the same thing, I think your hypothesis might be more of a prediction and you should elaborate on a proposed mechanism. I know this is unfinished so most of my advice would be stuff you were going to do anyway when you edited. I do think you might be headed towards a word count issue. You seem to be proposing doing two or even three experiments. I think the outline says do one experiment so you might just want to focus on the first knockdown one? Otherwise, your project sounds really interesting! Have fun finishing!
5) I think a figure about Gli regulation/mechanism would be really beneficial and help to replace/supplement the large block of text you have about Gli.
You’ve obviously worked really hard at your research project and it is looking really great! However, I would recommend cutting down some text (ex. some text in the experimental section) or breaking up the paragraphs to allow for easier reading.
6) Great detail in the background info and relevance. I like how you used figures to aid in the understanding of the information you are providing!