Rosalie's Biol 463 blog

> Given Disc Large 1 interacts with Gliotactin , protein candidates that may
> be involved in positioning Gliotactin on anakonda at the tricellular
> junction were identified. I would like to propose an experiment which
> tests whether CASK proteins, veli, uncharacterized Dmel amd PTEN2 proteins
> are necessarily in Gliotactin positioning.
>
>
> The rough experimental design part 1:
>
> Tag Gliotactin and Disc large with GFP and RFP. It is expected that the
> wild type control would exhibit co-localized signals at tricellular
> junctions in the wing imaginal disc. Using engrailed to drive GAL4 ,
> UAS-RNAis specific to candidate proteins would be expressed in the wing
> imaginal disc. Upon comparing the dorsum of the wing imaginal disc, where
> engrailed is known to express, to the rest of the wing imaginal disc
> which lacks engrailed expression, one can determine the necessity of
> candidate proteins in positioning. If the knock down of a protein causes
> delocalization of the disc large or gliotactin, it would suggest the
> protein to be necessary in Gliotactin positioning in the wing imaginal
> disc.