I had switched questions earlier in the term because I had wanted to look at at the long non-coding RNA H19. I was attracted to H19 for the wealth of information waiting to be discovered from the ample research that is currently in the literature. Little to my surprise (and chagrin), H19 proved to a really interesting topic that I’ve been finding it hard to narrow my question to something specific until now…
The questions that I have are in two parts: (May have to pick just one as I do think I am being too ambitious…)
- What is the developmental effect on kidney, of H19 microRNA products, mir-675-3p and mir-675-5p on the following transcription factors from the BMP family: BMP7, BMP11, and BMP4?
- Will the mir-675-3p and mir-675-5p produce the same results observed in skeletal muscles for Smad1 and Smad5 as they would in kidney cells in a transplantation experiment? In other words, are the microRNA products pre-programmed or can they adjust to their surroundings via environmental cues.