We are now offering public screening for mutations in Brassica napus using TILLING:
- Our population is derived from the doubled haploid line DH12075.
- We screen at least 1152 nutant lines, or screen until we have identified a putative null allele or several missense alleles.
- We usually screen amplicons of 1500 – 1800 base pairs, but this depends on the length of genomic sequence avaialble to design primers
- We obtain, on average, more than 20 mutations per gene (~45% missense, 5% nonsense, and 50% silent)
- The cost for TILLING of one region (1500 base pairs) is $5000 CAD.
- In order to make a request we require either genomic or cDNA sequence from a Brassica napus variety (preferably DH12075). If genomic sequence is provided, TILLING is generally faster because primer design is easier.
To make a request or for more information please contact us by E-mail.