Any mutagen that induces single basepair mutations or small deletions\/insertions is effective for TILLING. EMS (Ethylmethane Sulphonate) is the mutagen we have employed, for the following reasons:<\/p>\n
\u25cf Its effects have been well studied and it is known to generate almost exclusively G\/C to A\/T point mutations.<\/p>\n
\u25cf These mutations may lead to a complete or partial loss of gene function or, less frequently, to some other alteration of normal gene function.<\/p>\n
\u25cf Mutations are randomly distributed in the genome.<\/p>\n
\u25cf A high degree of mutation saturation can be achieved with a mutagen like EMS that does not cause a lot of collateral DNA damage.<\/p>\n
\u25cf For a review of the spectrum of mutations induced by EMS see: Greene EA, et al.<\/em>, 2003. Genetics 164:731-740.<\/p>\n","protected":false},"excerpt":{"rendered":" Any mutagen that induces single basepair mutations or small deletions\/insertions is effective for TILLING. EMS (Ethylmethane Sulphonate) is the mutagen we have employed, for the following reasons: \u25cf Its effects have been well studied and it is known to generate … Continue reading