Colby Song
3574 West Avenue
Vancouver, B.C. V5T 4H8
Phone: (778)-989-9898
Email: csong@gmail.com
Objective Research Technologist for STEMCELL Technologies R&D Division
Education University of British Columbia, Vancouver, BC
2010-present B.Sc in Biochemistry, expected graduation in May 2015
Employment
Jan-May 2014
UBC Department of Ophthalmology and Visual Sciences Moritz Lab
Co-op Student/Research Assistant
– Investigated mechanisms underlying photoreceptor cell death in Retinitis Pigmentosa
– Projects include monitoring endoplasmic reticulum stress caused by bP23H mutation in a transgenic Laevis model, and characterizing mutations
– Performed literature searches and carried out experiments using molecular biology techniques
– Interpreted and presented confocal microscopy data at regular lab meetings
May-Dec 2013
UBC Department of Chemical & Biological Engineering Research Group
Co-op Student/Research Assistant
– Investigated potential strategies directed towards purifying α-cellulose from bamboo
– Projects include developing kinetic model of hemicellulose degradation in bamboo, biomass sugar analysis and cellulase/xylanase treatments
– Conducted literature searches and designed experiments involving FTIR and enzymatic assays
– Interpreted and presented HPLC and FTIR data at lab meetings including a final report
– Assisted with the setup, maintenance and trouble shooting of HPLC
– Co-author in poster to be presented at Paptac Biorefinery Summit
Projects
2013
Cloning and Insertion of E.coli LacZ Gene into pET plasmids
– Prepared PCR master mix and proper negative and positive control samples for PCR reaction
– Checked the success of PCRs using agarose gel electrophoresis
– Generated chemically competent DHα coli cells with CaCl2 and performed transformation of these cell with newly generated plasmids on prepared LB-AMP plates
2013
HIS-Tagged Recombinant LacZ Protein Expression and Purification
– Induced expression of recombinant β-galactosidase in BL21 coli using IPTG as inducer
– Purified recombinant protein using metal chelate affinity chromatography with Ni-NTA
– Confirmed the success of recombinant protein isolation using SDS-PAGE and Dot Blot
– Quantified amount of protein isolated using ONPG and Bradford Assay and protein spectroscopy
Volunteer
2012-2013
Impact BC Basics for Health
Family Worker
– Demonstrated strong communication and interpersonal skills in assisting individuals and families with problems such as financial security, education and social supports
– Effectively organized client data base using MS Outlook and updated client profiles using objective style writing
Skills
Biochemistry techniques: Agarose Gel Electrophoresis, Column Chromatography, PCR, Plasmid Preparations, SDS-Page, DNA Spectrophotometry, Bradford Protein Assay, Restriction Enzyme Digests, Recombinant Protein expression and Purification, ONPG Assay, Protein Spectroscopy, Dot Blot
Chemistry techniques: reflux, extractions, purifications, multi-step organic synthesis, fractional distillation, recrystallization, titrations, gravimetric analysis
Characterization by IR, NMR, MS and GC
References Available Upon Request