Category Archives: News

Can Cleaner Pig Poop End Global Warming?

Pigs – photo from Wikimedia Commons

In short, no. However, it may be able to reduce the carbon footprint of the pork industry. You’re probably wondering what in the world pig manure has to do with climate change. Trust me, I was in the same position before embarking on some research of my own.

Piles of pig manure – photo from Geograph

Apparently pigs lack 3 important enzymes needed to digest the nitrogen and phosphorus in their feed. Nitrogen oxide, a dangerous climate pollutant, is thus released from the manure of these animals. Although it only makes up about 6% of all US greenhouse gas emissions, it can trap 300 times as much heat as carbon dioxide, according to EPA. Reducing nitrous oxide emissions could play a major role in reversing the effects of global warming.

2016 U.S. nitrous oxide emission, by source – Data courtesy of EPA

5% of nitrous oxide emissions are a result of manure. To tackle this source, scientists began looking into finding a way to enhance the digestion of feed in pigs in order to reduce the excretion of dangerous pollutants. Xianwei Zhang and colleagues at the South China Agricultural University considered genetically modifying their pigs to be better able to digest these nutrients. The findings, which were published in eLife, proved that the new generation of transgenic pigs did in fact release less nitrogen.

So how exactly were the pigs genetically engineered?

Firstly, the scientists looked at what pigs lacked to know what to modify. The enzymes that pigs needed for the digestion of nitrogen were b-glucanase, xylanase and phytase. Zhang and researchers attempted introducing genes required to make these enzymes into the genomes of the pigs by a process called Somatic Cell Nuclear Transfer, or more simply, cloning. The image to the left outlines the key steps.

Next, they determined how to make the digestion process as efficient as possible. By expressing the 3 bacterial enzymes in the animals’ salivary glands, they were able to ensure that digestion began in the mouth.

Now comes the unpleasant part. In order to test their methods, the team was required to measure nutrient levels in the pigs’ manure and urine. That’s right, they studied poop!

As predicted, fecal nitrogen outputs in the modified pigs were reduced by as much as 45.8%. The scientists also noted that due to an increase in nutrient digestion, the pigs were becoming fully grown a month earlier than their non-genetically modified peers.

By growing fast, requiring less food and producing fewer pollutants, the pigs developed by Zhang and her team could lead to a win-win situation for farmers and the environment. Now imagine the benefits if we apply similar methods to major sources of agricultural greenhouse gas emissions, such as cows and sheep!

Overcoming “The Wall”

If you’ve ever watched a marathon, you’ve probably seen some runners collapse just short of the finish line. Or you’re out on a run and it suddenly seems like you’ve completely run out of energy, despite your brain raring you to go. It’s almost as if the connection between your brain and your body has been severed. This is known as “Hitting The Wall”, or “Bonking” if you’re a cyclist.

Runners from the Dublin Marathon in 2013 – Photo from flickr

It was thought for a while that using up the body’s glucose reserves was the cause of this. Instead, a new study in Cell Metabolism surprisingly shows that “hitting the wall” actually happens when your brain cannot get access to sufficient glucose. While the muscles in our body can use fat or glucose as fuel, the brain can only use the latter.

When we hear the words “Progressive Training”, we often think of a training plan that increases in intensity and difficulty over time, therefore “improving our fitness” as we get used to longer and tougher bouts of exercise. For example, progressive training for marathon runners would involve increasing the total distance run per week over a period of time leading up to a marathon.

Endurance athletes often use GPS watches to track different aspects of their training such as distance, heart rate, cadence etc. – Photo from flickr

In reality, progressive training actually reprograms our muscles to burn less glucose and more fat while in use, thereby preserving it as an energy source for your brain. Research in the study focused on a transcription factor known as PPARδ (pronounced PPAR-delta). PPARδ triggers muscle composition changes in our body and “teaches” our muscles to consume fat as fuel instead of glucose. Progressive training gradually activates PPARδ.

In the first set of experiments in the study, researches at Lausanne Switzerland’s Ecole Polytechnique Federale genetically knocked out PPARδ in the muscles of mice. The mice were then put on treadmills and the effects of the lack of PPARδ were studied. Dr Michael Downes said “”When we did this and then ran those animals on a treadmill, we found that the genes that are normally induced by exercise failed to be induced.”

With this information, they then fed another group of mice a small molecule drug that activated PPARδ. These mice were able to run for a longer time (160 mins vs 270 mins) compared to the mice that had PPARδ deactivated – despite no progressive training to improv their endurance. By activating PPARδ within the mice, they were able to mimic progressive training.

For endurance athletes like myself, this discovery is revolutionary. No matter how experienced, a marathon runner takes requires approximately 16 weeks of progressive training to achieve a new target time. While the research is still in its preliminary stages, a way to combine the effects of both progressive training and PPARδ could take athletic performance to a whole new level.

However, these findings can be exploited by athletes wanting a competitive edge. An entire new can of worms is opened with regards to the ethics of chemically activating PPARδ in competitive athletic events like the Olympics.

Nonetheless, the best promise lies in being able to improve the endurance in people who are unable to naturally activate PPARδ through training. People who are suffering from Duchenne muscular dystrophy and cystic fibrosis to name a few, are often unable to get the exercise they need. This would eventually result in deterioration of their fitness. Chemically activating PPARδ in these people would allow them to enjoy the benefits of being fit without having to go through an intense training regime.

Meet My Two Biological Moms!

It’s 2018. Same-sex relationships aren’t anything out of the blue. However, if I bring up same-sex mating, then that’s crazy, right? Well, researchers have found a way to make this possible – in mice. Humans will just have to wait, but wouldn’t it be cool if other mammals that only had one sex remaining in their population could reproduce and save their species from going extinct? I’d say that would be pretty cool. 

“Mouse” by Liz Henwood. Image from Flickr.

On October 11, 2018, researchers from the Chinese Academy of Sciences led by scientist Qi Zhou published their groundbreaking work in Cell Stem Cell  showing how they bred healthy mice from two mothers. The baby mice developed normally to adulthood and even had offspring of their own.

I know what you’re thinking, if two female mice could do it, what about the males? Unfortunately, it didn’t work out the same. Baby mice developed from two biological fathers only survived for about 48 hours.

Normally, mammals can only sexually reproduce, so an egg from a mother and a sperm from a father is required. How is it even possible that two mothers were able to make healthy babies then? The answer: Genetic engineering.

“Baby Mice” by Hannah Nicklin. Image from Flickr.

The work required an egg from one female mouse and a special cell from the other female that carries half of the genetic information to make the baby, called the haploid embryonic stem cell. Simply fusing the egg and cell together didn’t cut it, so the scientists cut out several genes from the DNA of the haploid stem cell to make the necessary alterations for successful reproduction, and then fused it with the egg. Through trial-and-error gene-cutting, 210 embryos were developed, but only 29 mice lived.

Figure 1. Percentages of Mice Embryo that Survived being Produced from Two Mothers. Total Number of Embryos = 210. Image created by Sonia Sharma, the author.

As shown above in Figure 1, the numbers clearly indicate that this specific genetic engineering technique needs some work as embryo death rates are well above 80%. However, the work did succeed with impressive results seen in the surviving mice.

If similar techniques could be used to successfully have same-sex mammals reproduce in a population left with only one of the sexes, I’d be happy with that. It would be one step closer to preventing another sad extinction.

 

  • Sonia Sharma

Trimming the Fat: Is the Canadian Ban on Trans Fats Justified?

Canadian Minister of Health, Genitte Petitpas Taylor, announced one year ago that Canada would be banning the addition of trans fats in food, and that ban came in effect today. Officials predicted the ban is going to prevent 12000 deaths from heart disease over the next 20 years.

Heart disease is no laughing matter. According to the Canadian Ministry of Health, “About 1 in 12 (or 2.4 million) Canadian adults age 20 and over live with diagnosed heart disease” and “Every hour, about 12 Canadian adults age 20 and over with diagnosed heart disease die”. So, what can we do to beat heart disease? Exercising, drinking and smoking in moderation, and eating a healthy diet are what any doctor will suggest. When it comes to eating a healthy diet, trans fats are public enemy number 1, but why is that?

Trans fats contain cholesterol that is carried in the body by proteins. The most common are low-density lipoprotein (LDL) and high-density lipoprotein (HDL). These two lipoproteins bind to cholesterol to make it soluble in water so that it can move around our body. Unfortunately, LDL is also responsible for the plaques in our heart and arteries that contribute to heart disease. Knowing this, it’s a no-brainer to reduce the amount of LDL cholesterol we eat. Trans fats have a high proportion of LDL to HDL cholesterol relative to other fats, so avoiding them is the obvious choice. If you’re worried that your favourite treats will soon be off the shelf, there’s no need to worry.

Krispy Kreme Bacon Cheddar Cheeseburger – Courtesy Clay Caviness of Flickr

Protecting people is part of a government’s job, and the Canadian government should have taken action to protect Canadians from this health threat years ago. Canada has finally joined a global initiative to reduce trans fats in food, alongside the United States of America and the European Union. While our heel-dragging isn’t great for our health, we can at least rest easy knowing that food manufacturers in the USA and the EU have already had time to remove trans fats from their products, so we can keep buying them in Canada without interruption. While some alternatives to trans fats are a little more expensive, we can at least pay with our wallets rather than our health. I’ll raise a doughnut to that.

Automated Chemical Synthesis Machine: Reaction Optimization Has Never Been Easier!

Researchers in Massachusetts Institute of technology have developed an automated machine that can perform several chemical reactions in a short time to determine the most efficient route for synthesizing complex organic molecules such as various drugs and artificially synthesized natural products such as proteins and vitamins.

Researchers in the field of organic synthesis, spend weeks or months trying to come up with a synthetic route for a new complex organic molecule such as a new drug. These routes often are not efficient, so they spend even more time, trying to optimize the route so that the target molecule can be synthesized with high yields in large scales in industry. This new system can test and optimize a new synthetic route in about a day.

Synthesis of useful but complex organic molecules is a time-consuming process. Image from Pixabay

According to Timothy F. Jamison, head of MIT’s Department of Chemistry, in an interview with MIT News“our goal was to create an easy to use system that would allow scientist to come up with the best conditions for making their molecules of interest”. This would allow chemists to have more time to come up with new ideas that can be easily and efficiently tested.

This machine uses a continuous flow systemco-designed by Jamison a few years ago. In this system, chemical reagents flow through a series of tubes and at different points, new chemicals are added to the reaction. So, the machine performs a multi-step synthetic process continuously without the need to manually transfer the products of a reaction to be used as the starting materials of the next reaction.

So, the chemist comes up with a new synthetic route for a desired molecule and programs the machine with the reaction conditions. Then the machine uses an optimization program to explore different conditions and determines the reaction conditions that result in the highest yield.

This new system can test and improve a synthetic route for a complex organic molecule like a pharmaceutical in a day. Image from shutterstock

In my opinion, innovations like this can easily and rapidly improve synthetic organic chemistry so many useful natural organic molecules can be efficiently synthetized in industry. This means more reasonable prices for many drugs and important supplements.

The researchers have patented this new technology and published the results of the research as a journal article in Science.

 

Bioterrorism: An Impending Global Threat

Since antiquity, biological warfare decimated the opposition.  The Assyrians developed fungus that poisoned enemy forces while the Mongols threw fallen soldiers over city walls to spread disease. However, present day treaties and protocols have banned biological weaponry for military use.

Unfortunately, the modern age introduced a new threat: Bioterrorism.

Members of the Japan Ground Self-Defense Force’s Special Weapons Protection Unit (U.S. Marine Corps photo by Lance Cpl. Tyler S. Giguere/Released)

Security agencies around the world struggle to detect biochemical agents. Terrorists can easily create anthrax and smallpox to inflict mass hysteria in enclosed areas such as airports or schools.

Anthrax acts as a potent nerve agent (destroys the body’s nervous system), while smallpox can infect millions of people, killing 30% of those afflicted. Thus, governments have responded by creating defence organizations tasked with combating this emerging threat.

“He who fights with monsters should look to it that he himself does not become a monster.” – Nietzsche

To me, this quote best describes the proper way of fighting back. I believe using destructive weaponry to annihilate terrorists does not make us any better than them. A fine line must exist, that separates us from becoming the very monsters, we fear ourselves.

So far, military research has focused more on prevention over elimination of the enemy. Particularly, in the field of spectroscopy which studies how light interacts with physical objects.

A study done in 2011 by researchers from the Defense Science & Technology Laboratory United Kingdom developed PD-SORS (Point Detection – Spatially Offset Raman Spectroscopy), a new technique that can detect traces of urine hidden in containers from distances of up to 20 cm away. Very similar to the Tricorder from Star Trek (a portable scanning sensor).

SORS works like this: a laser beam fires from a box and hits an object (encased by a barrier) which then scatters around its container before returning back to the box for analysis. A variety of barriers were tested: ranging from paper envelopes, jiffy bags and complex chemical compounds. To great effect, the detector picked up urine traces in all different types of containers!

Raman spectra of drugs. Courtesy of Wikimedia Commons.

Raman spectra of drugs. Courtesy of Wikimedia Commons.

SORS serves a crucial role in defence because traditional Raman spectroscopy excels in transparent and open-air settings. However, most threats hide from the public eye. Terrorists will place dirty bombs in suitcases, jackets and even coffee cups!

The ability to detect sub-surface threats proves invaluable in thwarting potential terrorist attacks. This practical application will allow airport security to improve their efficiency and accuracy when screening for potential threats at checkpoints.

Fighting headstrong may have worked in ancient times, but bioterrorism will require a different strategy. The key lies in prevention, where the greatest victory requires no battle.

Octopi: They’re Just Like Us!

I grew up on a farm in land-locked Alberta. Apart from a couple of family vacations, the most experience I had with the ocean was watching fields of wheat break into waves on a correctly windy day. I have always been a bit hesitant when it comes to the Great Blue Sea. Its vastness and wide variety of occupants intimidate me. However, Vancouver’s close proximity to the Pacific has altered my mindset. Knowledge is power, and if I am able to understand bits of the ocean’s makeup, I will be less wary.

YouTube Preview Image

Link to Video of a Wheat Field in the Wind on Youtube

Researchers have published a paper which links humans and octopi through a common gene involved in social interaction that has been preserved independently in each lineage for over 500 million years. Their approach? A little trippy.

Chemical Structure of MDMA. Image courtesy of Erin Finnerty

Scientists from John Hopkins University and Josephine Bay Paul Center gave Octopus bimaculoides MDMA, the party drug also known as ecstasy, in an attempt to see its effects on social interaction. It is known that MDMA encourages prosocial behaviour in humans and mice, but invertebrates were never considered. Induced O. bimaculoides spend a significantly increased amount of time socially interacting with other octopi than when sober.

Chemical Structure of MDMA. Image courtesy of Erin FinnertyOctopuses were given the option to interact with an inanimate object, a social object (male or female octopus), or stay in the empty central area of a three-chambered tank. Untreated O. bimaculoides spend more time with the novel object than in the other tanks. When the social object is a female, subjects spend a larger amount of time in the social chamber than when the social object is a male. This shows a significant preference for female interaction.

These creatures are asocial and solitary, naturally interacting with others only for ecologically favourable purposes such as mating. MDMA increases serotonin levels in the body. Serotonin helps promote social interaction and therefore, positively influences sociality.

When soaked in an MDMA bath, O. bimaculoides prefers to spend time with male social objects than with the novel object. Their interactions are much more exploratory, and subjects use their entire body for extensive surface contact when under the influence. Effective MDMA doses were analogous with those of humans and rodents.

At the same time, the genome of O. bimaculoides was successfully sequenced to find the gene SLC6A4. This gene is known in humans to be involved with serotonin neurotransmission and transportation. O. bimaculoides carries the gene and the ability to be social, but the neural mechanisms are suppressed until ecologically necessary.

Photo of O. bimaculoides at Santa Monica Pier Aquarium in 2006. Photo courtesy of Jeremy Selan

Dr. Gül Dölen, the lead investigator, summarizes the research: “What our studies suggest is that certain brain chemicals, or neurotransmitters, that send signals between neurons required for these social behaviors are evolutionarily conserved.” She also suggests that more research need to be done in exploring the homology of these lineages as these results are preliminary.

I find it very comforting to know that there is a scientific correlation between humans and octopi. This does not mean you could convince me to go scuba diving tomorrow, but using my interest in genetics and ecology is an effective tactic!

The Bowel Movement: A Revolution in Treating Gut Disease

If you have ever suffered from an upset stomach, your doctor might have prescribed you an antibiotic to help you feel better. But repeatedly using antibiotics can make us more susceptible to severe forms of disease that antibiotics cannot treat. One such disease is caused by the bacterium Clostridium difficile and is characterized by symptoms ranging from mild diarrhea to rupturing of the bowel in extreme cases. Luckily, the future for treating such conditions doesn’t seem to be a complete stink as scientists believe we might be able to use our own poop to combat diseases of the gut.

Clostridium difficile bacteria are tiny inhabitants of your gut wall. They are about a million times smaller than a baseball bat and can only be seen with a microscope. (Image Source)

Your own poop? Yes. Call it a trans-poo-sion to appear cool and hip like today’s millennials.

A study published in March 2015, by scientists from Italian universities, has shown that fecal transplants are more effective in treating diarrhea caused by Clostridium difficile than one of humankind’s most powerful antibiotics. The findings come at an important time when newly emerging strains of antibiotic-resistant bacteria pose a huge risk for human health and health-related government expenditure.

The problem with overusing antibiotics is that they negatively impact the community of micro-organisms living in your gut, collectively known as the microbiome. The microbiome is important in digesting our food, but is also part of the disease-fighting immune system. Common thought has made us believe that removing bugs from our bodies can help us improve our health, but removing some of these competitors may allow for more dangerous strains of bacteria such as C. difficile to flourish.

There are more than 10 times as many microbes inside and on our bodies than there are human cells – The gut microbe community is one of the most complex. (Image Source)

Fecal transplants could be used to restore a damaged microbiome and allow the body to fight off an infection. While I was initially skeptical about the idea, it now seems reasonable – If a damaged microbiome promotes disease, then why not replace the microbiome itself? According to the scientists, infusing a patient with feces from a healthy donor can help replenish useful communities of microorganisms that may have previously been wiped out by an antibiotic.

In their study, the scientists administered either a treatment of vancomycin, a powerful antibiotic, or a fecal transplant to 39 patients suffering from recurring C. difficile infections. The results were astounding: a whopping 90% of patients treated by fecal transplants recovered from the infection, compared to only 26% of the patients who received an antibiotic. 

The promise of fecal transplants for treating gut diseases has had far-reaching effects, with companies investigating ways of making the treatment more accessible. OpenBiome, a non-profit company based in Boston, currently freezes poop samples to a low enough temperature to be packed into ready-to-take capsules. However unconventional, I believe that re-defining traditional pharmaceuticals is important in the odyssey of treating disease – As disease-causing organisms become smarter and more powerful, our treatments need to become more sustainable asides from being just effective. 

In opposition, skeptics argue that the long-term safety issues of using feces to treat disease are still unknown. But with all new treatments comes a probation period to unravel some of its problems. At present, the case for poop transplants seems more substantiated than the case against.

In this spirit, it might be a good idea to start thinking about saving your poop for a rainy day. 

Scientists Have Found Plastic-Eating Worm! Can They Save Our Environment?

Plastic pollution is one of the most serious environmental issues on earth. Annually, human release trillion of plastic wastes into the ocean without breaking down the plastic. I have seen a lot of documentary films talk about how marine animals and seabirds are being injured and killed by plastic pollution. Fortunately, scientist Dr. Federica Bertocchini and her colleagues from Cantabria in Spain have discovered an insect that can digest plastic bags.

Dr. Federica Bertocchini, who works at the Institute of Biomedicine and Biotechnology of Cantabria in Spain, is a beekeeper in her free time. She accidentally discovered the plastic-eating worms while she was cleaning beehives at her home. She found that the beehives were infested by caterpillar larvae of Galleria mellonella, commonly known as the wax worm.

Waxworm – photo from Wikimedia Commons

“The wax worms are a plague for beekeepers; I put the worms in a plastic bag and after a while, I realized that the bag was riddled with holes and the worms were all around in my flat,” said Bertocchini while she was in an interview with Roechling Stiftung.

The discovery made Bertocchini realize a possibility of plastic biodegradation by using a natural agent. Then Bertocchini and her research team decided to investigate whether if the wax worms actually degraded the plastic bag, or they just physically chewed it for escape. They gathered some wax worms in a plastic bag. After 12 hours, they noticed that the worms obviously reduced the mass of plastic bag. Then a further testing showed that the wax worm chemically converted the plastic into other materials, which proved that a wax worm is a possible approach for biodegradation.

Watch how waxworm breaks down the plastic bag from the following YouTube video:

YouTube Preview Image

Why is wax worm able to degrade the plastic bags? Bertocchini and her team conclude that may be due to the place where the wax worm lives: the beehives. Wax worm consumes beeswax and honey as food, and beeswax has a similar chemical structure as plastic. Thus, the wax worm can chemically digest the plastic. A more specific explanation of their research can be found in their paper in Current Biology.

The question now is whether if scientists can use wax worms to solve plastic pollution. In my opinion, it is irresponsible for scientists to directly use them in our environment. Although wax worms can break down plastic bags in a more natural way, wax worms are enemy to bees. No one can ensure that wax worms will only digest the plastic wastes, since they may turn to destroy the habitat of bees which will bring more environmental issues to us.

“The idea of actually using the caterpillars never crossed our mind,” says Bertocchini. “They are a plague for the environment, you cannot throw millions of worms in the environment, the equilibrium in nature must be respected.”

-Tina Sun

Overcoming “The Wall”

If you’ve ever watched a marathon, you’ve probably seen some runners collapse just short of the finish line. Or you’re out on a run and it suddenly seems like you’ve completely run out of energy, despite your brain raring you to go. It’s almost as if the connection between your brain and your body has been severed. This is known as “Hitting The Wall”, or “Bonking” if you’re a cyclist.

Runners at the Stockholm Marathon 2006 – Photo from flickr 

It was thought for a while that using up the body’s glucose reserves was the cause of this. Instead, a new study in Cell Metabolism surprisingly shows that “hitting the wall” actually happens when your brain cannot get access to sufficient glucose. While the muscles in our body can use fat or glucose as fuel, the brain can only use the latter.

The study shows that progressive training reprograms our muscles to burn less glucose and more fat while in use, thereby preserving it as an energy source for your brain. Research in the study focused on a transcription factor known as PPARδ (pronounced PPAR-delta). PPARδ triggers muscle composition changes in our body and “teaches” our muscles to consume fat as fuel instead of glucose. Progressive training gradually activates PPARδ.

In the first set of experiments in the study, researches at Lausanne Switzerland’s Ecole Polytechnique Federale genetically knocked out PPARδ in the muscles of mice. The mice were then put on treadmills and the effects of the lack of PPARδ were studied. Dr Michael Downes said “”When we did this and then ran those animals on a treadmill, we found that the genes that are normally induced by exercise failed to be induced.”

With this information, they then fed another group of mice a small molecule drug that activated PPARδ. These mice were able to run for a longer time (160 mins vs 270 mins) compared to the mice that had PPARδ deactivated – despite no progressive training to improv their endurance. By activating PPARδ within the mice, they were able to mimic progressive training.

While these findings can be exploited by athletes wanting a competitive edge, the best promise lies in being able to improve the endurance in people who are unable to naturally activate PPARδ through progressive training. People who have been sidelined after a serious accident or suffering from diseases that such as Duchenne muscular dystrophy and cystic fibrosis.