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Farming and Climate: Organic or Bust?

I previously mentioned that I have little knowledge of the ocean as I grew up in the Prairies. My understanding of crop science and farming is, however, probably more extensive than the average person’s. Despite this, my grandparents only taught me about conventional farming.

Photo of my grandparent’s farm in Sturgeon County, Alberta. Photo from Samantha Kluthe

Organic farming is gaining more and more popularity with consumers. USA Today reports that the organic food sales hit $43 billion in 2016. It’s important to be aware of organic farming benefits with respect to our global energy crisis. I am interested in how organic farming differs to conventional methods in terms of energy consumption, and if it is a viable alternative in meeting the global food demand.

Whole Foods Market in Union Square. Whole Foods has a large influence in the organic food market. Photo from Aspersions.

I found a study  on the energy efficiency of organic and conventional crop production with respect to two crop rotations. Researchers from the Universities of Manitoba and Saskatchewan discovered a significant interaction between rotation and management systems with respect to efficiency (output energy/input energy). Organic farming methods paired with integrated crop rotations are the most energy efficient. The energy input was 50% less in organic methods compared to conventional farming.

Crop rotation is the method by which farmers switch the type of crop they are growing on the same section of land every year. It seems counterintuitive to not always plant the crop with the highest selling price, but it leaves farmers with higher long-term yields. Although not as valuable, incorporating a legume-based field allows farmers to decrease their chemical fertilizer usage as nitrogen is naturally fixed. Weeds and insects are also discouraged as each new crop disrupts their life cycles and comes with its own biological variation.

The study took data from twelve years of farming with combinations of grain-based rotation and integrated rotation with conventional and organic styles of management. Grain-based rotations used wheat, peas and wheat flax (WPWF) as crops. Wheat alfalfa and alfalfa flax (WAAF) were the crops of the integrated rotation.

Figure 1. The total and output energy from 4 different farming styles. WPWF: Grain-based rotation, WAAF: Integrated rotation, C: conventional production, O: organic production. Data is from Hoeppner et. al (2006). Figure created by Elizabeth Porter.

Crop management and rotation style affect energy output independently. Figure 1 shows that integrated crop rotation produces a much larger output than grain-based. The alfalfa is able to fixate nitrogen, consequently suppressing weeds. The large difference in output energy is also because the crop types in WPWF and WAAF result in seeds and whole plants, respectively.

Figure 1 shows that the energy input is higher for conventional farming, regardless of crop rotation. This is because of the addition of fertilizer and pesticide to fields which increases fuel and machinery use.

The researchers concluded that organic farming is a much more energy efficient management system than conventional. It seems that it should be the direction that agriculture should step towards to prepare for the future energy crisis. However, the energy output is also higher for conventional style crops (Figure 1). It seems unlikely that organic farming will be able to keep food needs met, especially with the ever-rising global population.

From this, I am confident that scientists are headed in the right direction, but am still wary about spending 47% more on organic foods….

How much protein do we really need while in a caloric surplus?

The general public has always been confused as to which diets are the ideal fit to sustain health and longevity to live a long and disease-free life. Examples include diets that are high in protein, low in carbs, high in fat, low in fat, or high in carbs.

Grass-Fed beef. Creative Commons Attribution-Share Alike 2.0 Generic. https://commons.wikimedia.org/wiki/File:Eye_Fillet,_Grass-Fed_Beef.jpg

 

Athletes are an example of people who require extensive information surrounding fitness and nutrition and muscle building is usually required when starting out. Furthermore, there are underweight individuals who struggle to gain weight. In both cases a need is gain weight in a healthy way is called for. What defines healthy weight gain? Most athletes would mention that they would like to gain muscle rather than fat because increasing lean body mass will increase your metabolism since muscles are a metabolically expensive entity. Which macronutrient partitioning should be used for maximal muscle growth and minimal fat gain?

Creative Commons Attribution-Share Alike 4.0 International. https://commons.wikimedia.org/wiki/File:Protein-rich_Foods.jpg

A study published in the Journal of the American Medical Association took a single-blind randomized controlled trial which included 25 male and female volunteers with a body mass index between 19-30 and aged 18-35. The participants first consumed a diet (15% of energy from protein, 25% from fat, and 60% carbohydrates) for 13-25 days designed to yield information about their basal caloric needs. The following 8 weeks patients were fed 40% calories higher than their basal caloric requirements. There were three groups in this study 1) Fed a low protein diet (6% of energy from protein, 52% from fat, and 42% from carbohydrates) 2) Fed a moderate protein diet (15% of energy from protein, 44% from fat, and 41% from carbohydrates) 3) Fed a high protein diet (26% of energy from protein, 33% from fat, and 41% from carbohydrates).

The low protein group had significantly lower changes in weight and lean body mass than the other groups. The high protein group and the moderate protein group didn’t have statistically significant differences in weight change, accumulation of fat, and changes in lean body mass.

Figure 1. Body Composition Changes from 8 weeks of Overfeeding

The data shows that changes in body composition can be highly affected by protein intake since the amount of protein you should be consuming according to the American dietary Acceptable Macronutrient Distribution Range (ADMR) is 10%-30% calories coming from protein for adults. The low protein group was only taking in 5% of their calories from protein and the results show that this resulted in a loss of lean body mass which is uncharacteristic when you’re in a caloric surplus. This means that the group that had 5% energy from protein was likely in a state of negative nitrogen balance which means that muscle breakdown exceeded muscle synthesis and due to lack of essential amino acids being present to replace the muscle that is broken down from regular muscle movement, the result is a loss in lean body mass which is undesirable while gaining weight.

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The Link Between Weight and Puberty

In the 1700’s the average girl got her first period she was 16, 100 years later  girls got their first period at age 14.  Today, most girls start puberty at age 12.5, precocious puberty puts girls at risk for breast cancer, cardiovascular disease and type 2 diabetes later on in life. Additionally, girls who go through puberty earlier are more susceptible to low-self esteem and depression. The timing of puberty is determined by genetics but also mediated by nutritional and metabolic cues. Previous research has pointed to hormones in meat and chemicals in food as endocrine disruptors causing increased levels of estrogen earlier in development.

Estrogen Molecule-Wikimedia Commons

However, this October at Oregon’s Health and Science University, Alejandro Lomniczi explored the link between increased body weight and the early onset of puberty in female rats. The human genome has not changed drastically enough in the past 150 years to cause such a large change in the timing of puberty. Therefore, Lomniczi and his co-workers focused their research on epigenetics, a field of study that explores changes in gene expression rather than changes in the genetic code itself.

Overview of Puberty in Boys and Girls-Wikimedia Commons

In their study they raised overweight, average sized and lean female rats while searching for genes relating to the hypothalamus.  The hypothalamus is found on the lower part of the brain and controls reproductive development and hormone release. Lomniczi found a puberty activating gene within the hypothalamus called Kiss1 that interacts with Sirtuin 1, an enzyme that transmits body weight information to the brain. Sirtuin 1 binds to the promoter of the Kiss1 gene inhibiting transcription and delaying puberty.  In his study, Lomniczi found that overweight rats had less Sirtuin 1 in the hypothalamus which upregulated the Kiss1 gene causing female rats to go through puberty earlier than the lean and average weight rats. Average weight rats had higher levels of Sirtuin 1, delaying the expression of Kiss1 and the onset of puberty.

This epigenetic pathway makes sense evolutionarily, if the body senses extra fat and calories it believes that there is enough nutrient sources to support a baby, triggering reproductive maturation. Consequently, girls who are underweight or even anorexic experience delayed puberty or stop menstruating because of increased levels of Sirtuin 1.

With these established links between weight and precocious puberty the only thing parents can do to prevent this, is ensuring that children are receiving proper nutrition and daily exercise.  Puberty in itself is an emotional time for both boys and girls, going through the process even earlier than their peers can have detrimental effects on on mental health and self-esteem.

-Priya Baichoo

Plan “Bee”

“If the bee disappeared off the face of the Earth, man would only have four years left to live. No more bees, no more pollination, no more plants, no more animals, no more man.” There is no easy way to take the sting out of Albert Einstein’s words.

Is this really the case? With our advancements in technology, why don’t we simply replace bees with immortalized robotic ones? That’s exactly what scientists did this past September at Delft University of Technology.

The DelFly Nimble, an agile insect-inspired robot from TU Delft by MatejTU. Image from Wikimedia Commons. CC BY-SA 4.0

Through the Delfly project, Karásek et. al created a free-flying robot, the DelFly Nimble, able to mimic the flight maneuvers of flies. This robotic model has a wingspan of 33 cm and weighs 28.2 g, which is 55 times bigger than a fruit fly. Reaching speeds up to 15 mph, the robot can fly a distance of 1 km in 5 minutes on a single battery charge.

Over the past decade, the decline of bees has been a major concern (Graph 1). For every action, there is a consequence. Farming advances, including pesticides and industrial agriculture, have negatively impacted bees. A 2015 UN report stated that 37% of bee populations are declining. Why should we care about this decline? Bees are the chief pollinator of our crops. Our daily diet consists of 40% produce (Graph 2) and 35% of crops produced are dependent on pollination (Graph 3). More importantly, essential crops, that are the primary source of vital nutrients and vitamins, are found within this category.

Graph 1. Number of US Domesticated Honeybee Colonies. Image created by Teresa Howard, the author.

Graph 2. Global Daily Food Consumption per Person in 2011. Image created by Teresa Howard, the author.

Graph 3. Crop Pollination Dependence. Image created by Teresa Howard, the author.

So what could solve this pressing problem? Matěj Karásek presents one possible solution with the DelFly Nimble. “The use we see for this is pollination in greenhouses. The bee is under threat due to our farming methods and we don’t know what their future will be. This is one solution.”

I believe it’s always good to have a Plan “Bee”, like the one Karásek suggests, to combat these problematic situations. However, we shouldn’t give up hope on the bees just yet.

Major contributors to the decline in the bee population include pesticides, industrial agriculture, parasites/pathogens, and climate change. To save the 20,000 species of bees, we can all help in little ways by planting bee-friendly flowers and avoid using pesticides.

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Youtube: TED Talk about why bees are disappearing

Now, this is all dandy, but will you actually make an effort? Each one of us needs to make a resolution to combat this decline and not rely on someone else. Some simple solutions, like the ones Dr. Spivak stated in her Ted Talk, can make a big impact on the bee population if all of us chip in.  

Bumble Bee on Purple Cone Flower. Attribution: http://www.ForestWander.com. Image from Wikimedia Commons. CC BY-SA 3.0

As of today, the Delfly project is still in development, making adjustments to the robot’s dimensions and battery life. Consequently, it may take awhile before the robotic bee “takes flight” as an effective pollinator. In the meantime, let’s all make an effort to bring back the bees.   

-Teresa Howard

Liquid metal make toxic water drinkable?

The freshwater crisis has been a serious global problem for years. There is 70 percent of the world covered by water while there’s only 2.5 percent of it is fresh. What if I tell you now there is a way we can transform from toxic water with heavy metal ion into safe drinking water?

Credit: © Vasily Merkushev / Fotolia

Credit: © Vasily Merkushev / Fotolia

In 2018 September 21, researchers in UNSW and RMIT funded by The ARC Centre for Future Low-Energy Electronics Technologies (FLEET) published an efficient and revolutionary method to transform water with heavy metal ion into drinking water by filtering the water through aluminum oxide nano-filters. It indicates that the water source recycle will be improved further with the massive production of this portable and low-cost filter paper.

The research shows that the liquid metal-based filter paper can excellently separate the oil and heavy metal ions(mainly lead ion), which highly improved water source recycle system.  The lead ion has a strong affinity to the aluminum oxide surface while the water doesn’t react with it. Thus, safe drinking water is obtained after the filtration.

The method of making this aluminum oxide nano-filters are quite simple and more importantly, cheap. First thin sheets and nanofibers of boehmite while annealing. The researchers then add of aluminum into gallium alloy in room temperature, then they expose the melt to liquid water or water vapor and aluminum oxide will be synthesized on the alloy’s surface.

Fortunately, The researchers decided to release their study to the public, which means that people all over the world are able to produce the same aluminum oxide nano-filters free and increase the quality of fresh water worldwide.

The researcher also indicates that more research about using liquid metal as the base material is needed in the future.

 

 

Contagious Yawning

Creatures’ brains relax and refresh after yawning, and they will better to continue work. However, have you ever notice that you start to yawn after the person next to you yawned even if you don’t feel tired? Or after you yawn, the person next to you start to yawn. Is yawning contagious? Do the sandmen fly to one person to let them yawn and then fly to another one? The answer is no, sandmen are not real.

A yawning cat — Credit to the Author

Instead, contagious yawning is natural, even animals have contagious yawning. One research team conducted an experiment which volunteers were asked to yawn after watching a yawning video. The research indicated that the brain’s primary motor cortex automatically triggers the relax. This reaction can cause creatures to yawn.

The other word to explain this behavior is empathy. Empathy can be defined as the ability to sense others’ emotion or feeling. One person can understand and feel another’s emotion when he/she yawns. Afterward, that person who sensed another’s emotion starts to imitate him/she to feel his/her feeling. Yawning has the same function, this is why when you observe a person yawn, you will start to yawn.

Contagious around different animals — image from baillement.com

However, contagious yawning does not always happen. A science writer Sam Kean indicated that human can pick up contagious yawning after the age of 4 to 5, whereas the small kids cannot have that behavior. Also, the chance of having someone yawn back might higher if you yawn in front of your family or relatives.

Another research shows contagious yawning might exist in brain substrates that related to self-recognition and mental state. These brain substrates control contagious yawning. In that research, the researchers observed subjects’ behavior while they watch videos about yawning and find out that most of the normal volunteers started to yawn involuntary. However, most of the schizophrenic volunteers did not. Therefore, schizophrenic patients who lack recognition ability and the mental state attribution to others show little or no contagious yawning.

Although some of the research can figure out the reason for contagious yawning, it remains an unexplained mystery. Contagious yawning may also relative to temperature and further research should be done to solve the problem.

–Catherine Wu

Octopi: They’re Just Like Us!

I grew up on a farm in land-locked Alberta. Apart from a couple of family vacations, the most experience I had with the ocean was watching fields of wheat break into waves on a correctly windy day. I have always been a bit hesitant when it comes to the Great Blue Sea. Its vastness and wide variety of occupants intimidate me. However, Vancouver’s close proximity to the Pacific has altered my mindset. Knowledge is power, and if I am able to understand bits of the ocean’s makeup, I will be less wary.

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Link to Video of a Wheat Field in the Wind on Youtube

Researchers have published a paper which links humans and octopi through a common gene involved in social interaction that has been preserved independently in each lineage for over 500 million years. Their approach? A little trippy.

Chemical Structure of MDMA. Image courtesy of Erin Finnerty

Scientists from John Hopkins University and Josephine Bay Paul Center gave Octopus bimaculoides MDMA, the party drug also known as ecstasy, in an attempt to see its effects on social interaction. It is known that MDMA encourages prosocial behaviour in humans and mice, but invertebrates were never considered. Induced O. bimaculoides spend a significantly increased amount of time socially interacting with other octopi than when sober.

Chemical Structure of MDMA. Image courtesy of Erin FinnertyOctopuses were given the option to interact with an inanimate object, a social object (male or female octopus), or stay in the empty central area of a three-chambered tank. Untreated O. bimaculoides spend more time with the novel object than in the other tanks. When the social object is a female, subjects spend a larger amount of time in the social chamber than when the social object is a male. This shows a significant preference for female interaction.

These creatures are asocial and solitary, naturally interacting with others only for ecologically favourable purposes such as mating. MDMA increases serotonin levels in the body. Serotonin helps promote social interaction and therefore, positively influences sociality.

When soaked in an MDMA bath, O. bimaculoides prefers to spend time with male social objects than with the novel object. Their interactions are much more exploratory, and subjects use their entire body for extensive surface contact when under the influence. Effective MDMA doses were analogous with those of humans and rodents.

At the same time, the genome of O. bimaculoides was successfully sequenced to find the gene SLC6A4. This gene is known in humans to be involved with serotonin neurotransmission and transportation. O. bimaculoides carries the gene and the ability to be social, but the neural mechanisms are suppressed until ecologically necessary.

Photo of O. bimaculoides at Santa Monica Pier Aquarium in 2006. Photo courtesy of Jeremy Selan

Dr. Gül Dölen, the lead investigator, summarizes the research: “What our studies suggest is that certain brain chemicals, or neurotransmitters, that send signals between neurons required for these social behaviors are evolutionarily conserved.” She also suggests that more research need to be done in exploring the homology of these lineages as these results are preliminary.

I find it very comforting to know that there is a scientific correlation between humans and octopi. This does not mean you could convince me to go scuba diving tomorrow, but using my interest in genetics and ecology is an effective tactic!

Should Some Breads be Age Restricted?

What if I were to tell you that every time you had consumed bread from a bakery, you were also consuming alcohol as well?

Although it is not recent news, a common way for bakers to create loaves of bread is to add yeast into their dough to make it rise. This idea comes from a famous french chemist named Louis Pasteur, who discovered the process of fermentation in 1857. In this process however, ethanol (the type of alcohol found in beer and other alcoholic beverages) is produced as a product. Is bread really safe to eat without the feeling of intoxication?

The short answer: Yes.

The process of fermentation involves converting one molecule of glucose into two molecules of both ethanol and carbon dioxide in the absence of oxygen.

The Process of Ethanol Fermentation

According to chemical engineer/data scientist Josh Velson,

“However, during the baking process, most of the alcohol in the dough evaporates into the atmosphere. This is basically the same thing that happens to much of the water in the dough as well.”

So no worries, bread is perfectly fine to eat. If bread is one of your favourite things to eat like me, this feeling of relief is the best thing since sliced bread.

Infinite Electricity: Gut Microbes Light the Way

As Einstein said, “Everything is energy and that’s all there is to it.” That’s exactly what scientists uncovered when studying microbes in the human gut.

At a time when governments are trying to discover renewable, clean sources of energy, the solution may be closer than most people think! Following a gut feeling, researchers discovered a microbial species that can generate electricity useful as a source for microbial fuel cells.

On Wednesday, the journal Nature, published findings from a University of California research team that discovered electron-generating bacteria found in the human gut capable of creating electricity. Light et al. showed that the gram-positive bacterium, Listeria monocytogenes, is able to make energy through extracellular electron transfer (EET) in the gut.

Listeria monocytogenes
By By Elizabeth White

EET is a process similar to the electron transfer pathway found in respiration. However, in EET, electrons are transferred to a final acceptor on the exterior of the cell, not the interior. The mechanism of EET is well known for gram-negative microbes, such as Shewanella oneidensis. Residing in an environment rich in minerals, S. oneidensis uses EET to transport electrons to an acceptor outside of the cell, creating accessible energy. By mutating L. monocytogenes, Light et. al identified key proteins in the EET transport pathway, but not the terminal electron acceptor.

Why was this exciting news? L. monocytogenes employs a simpler EET pathway using fewer electron transfer steps than gram-negative bacteria, opening up a new avenue to create potentially more effective microbial fuel cells. However, it’s important to note that the EET pathway isn’t vital for the survival of L. monocytogenes; it is simply more efficient in producing energy when the bacteria is in a nutrient-rich environment. Thus, I think that despite the existence of a simpler EET pathway for gram-positive bacteria, scientists would be wise to not give up on researching the gram-negative EET pathway since the conditions in which gram-positive bacteria use EET may be constrained to certain environments.  In addition, researchers have not yet determined which EET pathway, gram-negative or gram-positive, results in the creation of more energy, which is another reason for study into both EET pathways to continue.

Genomic studies by Light et. al, have identified a multitude of gram-positive bacteria with the potential of having EET proteins, indicating that the EET pathway of electrical production is evolutionarily and environmentally diverse, and may be present in currently undiscovered microbes. There may be a wide range of microbes waiting to be investigated to discover answers to my questions and make efficient microbial fuel cells.

Schematic of a microbial fuel cell. Microbes facilitate the oxidation of fuel in order to drive a current. By Bretschger O, Osterstock JB, Pinchak WE, Ishii S, Nelson KE

Discovering the EET mechanism of gram-positive bacterium, L. monocytogenes, has opened up the possibility of creating another form of microbial fuel cells. Unfortunately, I think the replacement of gram-positive bacteria in microbial fuel cells over gram-negative bacteria will continue to be unfeasible until the terminal electron acceptor in the EET pathway is identified along with the factors that affect the expression of the EET pathway in gram-positive bacteria.

-Teresa Howard

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Is BPA-Free Safe for Me?

I often find myself gravitating towards products that have labels such as “organic”, “cruelty-free”, “free-range”, etc. stuck to their packaging. It’s comforting to know I am opting for a product that is beneficial to either my health, the environment, or both. That’s why when I’m faced with the option to purchase a water bottle that is BPA-free, versus one that is not, 10 times out of 10 I buy the former. For years now I believed this choice was protecting me from the harmful effects of bisphenol A, a chemical known to disrupt reproduction in mice. Much to my surprise, this belief was shattered by a recent article in Current Biology that revealed that the replacement bisphenols are potentially just as harmful.

BPA-free plastic water bottles – photo from Pixabay

Patricia Hunt, a biologist at Washington State University who first discovered the damaging effects of BPA 20 years ago, has uncovered data that mirrors those findings. This new study shows that BPS, a replacement bisphenol, is causing abnormalities in the eggs and sperm of mice. Essentially, BPA-free plastics could still be causing the same adverse effects in humans that led us to ban BPA so many years ago.

The findings were uncovered much in the same way as the BPA discovery decades ago. While conducting unrelated research, Hunt noticed changes in the data collected from her lab mice. Specifically, she found abnormalities in their eggs and low sperm counts. “Given our previous experience with BPA leaching from polycarbonate cages and water bottles,” states Hunt, “damaged materials were an obvious suspect”.

Upon analysis of the plastic cages that the mice were being kept in, Hunt and her team discovered that the inner surfaces to which the mice were being exposed had traces of BPS. The compound, which has replaced BPA in many household items, was being released from the plastic from everyday wear and tear. Notice the similarities between the two chemicals in the images below.

Chemical structure of BPA – photo from Wikimedia Commons

Chemical structure of BPS – photo from Wikimedia Commons

To further investigate the issue, Hunt designed experimental studies in which mice were exposed to several replacement bisphenols. The effects were observed by looking at the number of DNA points where chromosomes had been reshuffled, known as MLH1 foci. This data was telling as MLH1 foci is indicative of the degree of abnormality. Females who were found to have high numbers of MLH1 foci were producing abnormal eggs. On the other hand, males who had low numbers experienced an increase in cell death during sperm creation.

The thought that plastic, a material that we use daily, could be affecting our reproductive health is extremely concerning. The results from Hunt’s most recent studies are remarkably similar to those seen years ago with BPA and raise the question if bisphenols as a class should be eliminated. I hope an alternative replacement in plastics will soon be discovered so that we can still enjoy the diverse benefits of this material.