Tag Archives: genetic engineering

Can Cleaner Pig Poop End Global Warming?

Pigs – photo from Wikimedia Commons

In short, no. However, it may be able to reduce the carbon footprint of the pork industry. You’re probably wondering what in the world pig manure has to do with climate change. Trust me, I was in the same position before embarking on some research of my own.

Piles of pig manure – photo from Geograph

Apparently pigs lack 3 important enzymes needed to digest the nitrogen and phosphorus in their feed. Nitrogen oxide, a dangerous climate pollutant, is thus released from the manure of these animals. Although it only makes up about 6% of all US greenhouse gas emissions, it can trap 300 times as much heat as carbon dioxide, according to EPA. Reducing nitrous oxide emissions could play a major role in reversing the effects of global warming.

2016 U.S. nitrous oxide emission, by source – Data courtesy of EPA

5% of nitrous oxide emissions are a result of manure. To tackle this source, scientists began looking into finding a way to enhance the digestion of feed in pigs in order to reduce the excretion of dangerous pollutants. Xianwei Zhang and colleagues at the South China Agricultural University considered genetically modifying their pigs to be better able to digest these nutrients. The findings, which were published in eLife, proved that the new generation of transgenic pigs did in fact release less nitrogen.

So how exactly were the pigs genetically engineered?

Firstly, the scientists looked at what pigs lacked to know what to modify. The enzymes that pigs needed for the digestion of nitrogen were b-glucanase, xylanase and phytase. Zhang and researchers attempted introducing genes required to make these enzymes into the genomes of the pigs by a process called Somatic Cell Nuclear Transfer, or more simply, cloning. The image to the left outlines the key steps.

Next, they determined how to make the digestion process as efficient as possible. By expressing the 3 bacterial enzymes in the animals’ salivary glands, they were able to ensure that digestion began in the mouth.

Now comes the unpleasant part. In order to test their methods, the team was required to measure nutrient levels in the pigs’ manure and urine. That’s right, they studied poop!

As predicted, fecal nitrogen outputs in the modified pigs were reduced by as much as 45.8%. The scientists also noted that due to an increase in nutrient digestion, the pigs were becoming fully grown a month earlier than their non-genetically modified peers.

By growing fast, requiring less food and producing fewer pollutants, the pigs developed by Zhang and her team could lead to a win-win situation for farmers and the environment. Now imagine the benefits if we apply similar methods to major sources of agricultural greenhouse gas emissions, such as cows and sheep!

Meet My Two Biological Moms!

It’s 2018. Same-sex relationships aren’t anything out of the blue. However, if I bring up same-sex mating, then that’s crazy, right? Well, researchers have found a way to make this possible – in mice. Humans will just have to wait, but wouldn’t it be cool if other mammals that only had one sex remaining in their population could reproduce and save their species from going extinct? I’d say that would be pretty cool. 

“Mouse” by Liz Henwood. Image from Flickr.

On October 11, 2018, researchers from the Chinese Academy of Sciences led by scientist Qi Zhou published their groundbreaking work in Cell Stem Cell  showing how they bred healthy mice from two mothers. The baby mice developed normally to adulthood and even had offspring of their own.

I know what you’re thinking, if two female mice could do it, what about the males? Unfortunately, it didn’t work out the same. Baby mice developed from two biological fathers only survived for about 48 hours.

Normally, mammals can only sexually reproduce, so an egg from a mother and a sperm from a father is required. How is it even possible that two mothers were able to make healthy babies then? The answer: Genetic engineering.

“Baby Mice” by Hannah Nicklin. Image from Flickr.

The work required an egg from one female mouse and a special cell from the other female that carries half of the genetic information to make the baby, called the haploid embryonic stem cell. Simply fusing the egg and cell together didn’t cut it, so the scientists cut out several genes from the DNA of the haploid stem cell to make the necessary alterations for successful reproduction, and then fused it with the egg. Through trial-and-error gene-cutting, 210 embryos were developed, but only 29 mice lived.

Figure 1. Percentages of Mice Embryo that Survived being Produced from Two Mothers. Total Number of Embryos = 210. Image created by Sonia Sharma, the author.

As shown above in Figure 1, the numbers clearly indicate that this specific genetic engineering technique needs some work as embryo death rates are well above 80%. However, the work did succeed with impressive results seen in the surviving mice.

If similar techniques could be used to successfully have same-sex mammals reproduce in a population left with only one of the sexes, I’d be happy with that. It would be one step closer to preventing another sad extinction.

 

  • Sonia Sharma