Dear Valued Customers,
One of our customers has asked us a mouse genotyping which has a transgene. They used to check the existence of the transgene with two kinds of primers. But it tells them just only positive or negative of that target transgene.
On the other hand, they also need to know “+/+” or “+/-” without designing new primers.
Yes, we do the Copy Number Assay, which allow us to tell “+/+ (two copies)” for “+/- (one copy)” in such a case. That is based on the TaqMan assay with a reference gene to see the difference of the copy number.
If you have similar situation to them, contact us and we may be able to assist your mouse genotyping, too.
Thanks,
Genotyping Facility