Dear Valued Customer,

We have had an inquiry from one of potential valued customers in BC Cancer Research Centre, about one of our services, Speed Congenics Mouse Screening.

The screening is performed by TaqMan based analysis. The genomic DNAs extracted from ear punches are run on the OpenArray Real-Time System, Life Techonologies. This service is designed just for checking the background your mice and we do not have any reference or scientific papers performed by our service available.

[mouse strain]
Currently the TaqMan based chip for Black6 and 129 only.
Each mouse strain with around 120 SNPs, 5 to 7 SNPs in each chromosom, are checked with your mice genomic DNA.
The SNPs in the chip were chosen at random by a Software, Mouse SNP Browser, Life Techonologies, by ourselves.

[number of samples]
There are up to 20 mice/chip are available to check. In the case of the duplicate, the capacity has up to 10 mice.

[Cost]
$2,000/chip (up to 20 samples a plate)

[Samples]
Just ear punches. We extract genomic DNAs.

[Turnaround]
Within five business days and email back to you with the document.

If you have any question, please feel free to ask us!!

Save time and focus projects,

Thanks,

Genotyping Facility

Dear Valued Customers,

One of our customers has asked us a mouse genotyping which has a transgene. They used to check the existence of the transgene with two kinds of primers. But it tells them just only positive or negative of that target transgene.

On the other hand, they also need to know “+/+” or “+/-” without designing new primers.

Yes, we do the Copy Number Assay, which allow us to tell “+/+ (two copies)” for “+/- (one copy)” in such a case. That is based on the TaqMan assay with a reference gene to see the difference of the copy number.

If you have similar situation to them, contact us and we may be able to assist your mouse genotyping, too.

Thanks,

Genotyping Facility

Dear Valued Customer,

We have had an inquiry from one of potential valued customers in the University of Calgary. And they asked what is included in the cost of the PCR-based Genotyping Service.

We perform it from the genomic DNA extraction through the PCR and analysis on the gel electrophoresis. The cost includes all the processes described above.

The customers just send us ear or tail samples. If you have any question, please feel free to ask us!!

Save time and focus projects,

Thanks,

Genotyping Facility

Dear Researchrs,

We have been providing the PCR-based genotyping services since 2004 at the Biomedical Research Centre on UBC campus. Services are covered from the disease-model mice genotyping to the pathogen detection in the mice.

The cost is very competitive and the turnaround is five business days.

What you need to bring is just your sample and we extract the genomic DNA to be ready for the genotyping.

Visit our website for the detail at http://www.brc.ubc.ca.

Regards,

Genotyping Facility