The challenge of developing a novel, testable question

You have been working on developing a novel, testable question on a gene
regulation-related topic. What is the most challenging aspect of it and why?

The most challenging part of developing a specific, testable question, was identifying whether the results of the experiment would have an impact on the research field.
As a university student, not yet having been part of the research world, I find it difficult to distinguish between highly relevant and important data, and data of lesser significance.
I feel I don’t have any experience in developing projects, deciding which experiments are important and which are dispensable.

Figuring out if my question had already been answered by previous published experiments took a lot of time. I concluded that it had not been, and so I thought: Have I actually identified a novel and relevant question? Or is the reason no one has investigated it because it is of no significance at all?

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