{"id":43,"date":"2016-11-11T14:51:19","date_gmt":"2016-11-11T21:51:19","guid":{"rendered":"https:\/\/blogs.ubc.ca\/lai463\/?p=43"},"modified":"2016-11-19T12:29:49","modified_gmt":"2016-11-19T19:29:49","slug":"techniques-presentation-crisprcas9","status":"publish","type":"post","link":"https:\/\/blogs.ubc.ca\/lai463\/2016\/11\/11\/techniques-presentation-crisprcas9\/","title":{"rendered":"Techniques Presentation: CRISPR\/Cas9"},"content":{"rendered":"<p>Link to the write-up and presentation <a href=\"https:\/\/blogs.ubc.ca\/lai463\/files\/2016\/11\/1-CRISPR.pdf\">CRISPR\/Cas9 Write-up and Presentation<\/a><u>.<\/u><\/p>\n<p>Looking back, I am glad that I had the opportunity to learn more about CRISPR through the Techniques Caf\u00e9, especially because in Learning Journal #1, I had expressed interest in learning about CRISPR in BIOL 463. After the Techniques Caf\u00e9, I received more than what I had hoped for in terms of learning about CRISPR. Instead of just learning about CRISPR, I taught others about CRISPR, which required me to have a solid understanding of it myself in order to effectively talk about the technique. Taking this into consideration, I believe that I learned a lot more from presenting CRISPR than I would have if I had simply listened to another group teach me about CRISPR. Overall, it was a satisfying experience to both learn about something that I <em>wanted<\/em> to learn about and learn it really well.<\/p>\n<p>If I were to test a person\u2019s understanding of how CRISPR\/Cas9 works, I would ask: Why is a DNA template necessary to allow genome editing to occur through CRISPR\/Cas9? In an answer, I would expect the person to acknowledge that without a DNA template, double-stranded breaks in the DNA will lead to non-homologous end joining, which results in the incorporation of random bases, and thus random mutations. Purposeful genome editing involves specific modification of sequences, rather than random mutagenesis. Therefore, a DNA template is necessary to allow specific modification of target DNA sequences.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Link to the write-up and presentation CRISPR\/Cas9 Write-up and Presentation. Looking back, I am glad that I had the opportunity to learn more about CRISPR through the Techniques Caf\u00e9, especially because in Learning Journal #1, I had expressed interest in &hellip; <a href=\"https:\/\/blogs.ubc.ca\/lai463\/2016\/11\/11\/techniques-presentation-crisprcas9\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":43090,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[943,2139,1729269],"tags":[],"class_list":["post-43","post","type-post","status-publish","format-standard","hentry","category-portfolio","category-reflection","category-techniques-presentation"],"_links":{"self":[{"href":"https:\/\/blogs.ubc.ca\/lai463\/wp-json\/wp\/v2\/posts\/43","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.ubc.ca\/lai463\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.ubc.ca\/lai463\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.ubc.ca\/lai463\/wp-json\/wp\/v2\/users\/43090"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.ubc.ca\/lai463\/wp-json\/wp\/v2\/comments?post=43"}],"version-history":[{"count":3,"href":"https:\/\/blogs.ubc.ca\/lai463\/wp-json\/wp\/v2\/posts\/43\/revisions"}],"predecessor-version":[{"id":52,"href":"https:\/\/blogs.ubc.ca\/lai463\/wp-json\/wp\/v2\/posts\/43\/revisions\/52"}],"wp:attachment":[{"href":"https:\/\/blogs.ubc.ca\/lai463\/wp-json\/wp\/v2\/media?parent=43"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.ubc.ca\/lai463\/wp-json\/wp\/v2\/categories?post=43"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.ubc.ca\/lai463\/wp-json\/wp\/v2\/tags?post=43"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}