During the DNA replication process, the DNA and proteins that form nucleosomes are disassembled and then reassembled exactly on the newly-replicated DNA. Histone chaperones are a broad class of proteins that deposit histones on the DNA, but which of these assist in the reassembly of chromatin in the newly-replicated DNA is unknown. Some proteins involved in DNA replication also have similar functions to these histone chaperones, which means that the replisome itself may mediate the replacement of histones after replication. My research question hopes to cover:

1. Which proteins are responsible for the deposition of histones and reassembly of nucleosomes immediately following DNA replication?

1. I learned more about chromatin architecture, and how cis-factors do not necessarily have to be close to each other in sequence, as long as they are in the same topologically-associated domain (TAD). Moreover, I also learned about different techniques that are used to study chromatin architecture and TADs (such as 3C and its derivatives), and I find all the progress being made in this field to be quite interesting.
2. These fit nicely into my learning objective of how proteins can regulate genes, and moreover how one protein can regulate different genes. If genes are in the same TAD, they can be regulated by the same trans-acting factors as well, and boundary elements that barrier this TAD from others can prevent those trans-acting factors from acting on other genes instead.
3. I think the most challenging part of the course so far has been learning what to directly conclude from experimental data, and how to analyze data. I am surprised by this as well as not too many of my other courses rely heavily on reading real papers, and instead focus on hypothetical data sets for experimental data.

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1. I would like to learn more about epigenetics and how else hereditary information can be passed on from generation to generation without affecting the gene sequence. I would also like to learn more about the interactions between DNA and proteins, and how these proteins affect regulation of different genes and how one protein can regulate multiple genes.

2. I believe that resisting distractions may be the most difficult for me in this context, as I have many other involvements on campus, and I sometimes lose track of what to do.

3. I do think that this reflection is a form of self-regulated learning, as it involves analyzing previous situations and the current situation alike, and developing a plan of action from there that will hopefully be an improvement from previous attempts.