Category Archives: Biological Sciences

Dengue Fever Virus: What Is it?

Warning for those who suddenly begin to feel fatigued, nauseous, and sport a severe headache! This is a warning sign that the mosquito bites you received on your trip through a tropical region a few months ago may have introduced a deadly virus into your body. At this point, you could be infected with Dengue fever virus.

A map detailing the prevalence of Dengue virus back in 2005. From Wikimedia.

 

According to an article published by Anne Gubler in Trends in Microbiology, Dengue fever virus originated from the tropical regions of the Earth during the 18th to 19th centuries. The virus is mainly spread through a species of mosquito, referred to as Aedes aegypti, which still populate the tropical and some subtropical regions today. However, as human population continues to increase in these areas, the risk for another epidemic outbreak increases. This is due to the ease at which a mosquito can travel from host to host, spreading the virus at a much higher speed. Recently, outbreaks of Dengue have intensified in all tropical regions resulting in an increase of reported infections and deaths. This makes me extremely cautious of visiting countries that are infested with mosquitos carrying Dengue and I’d like to spread some information about what it is.

 

General cross-section view of a Dengue virus. From Wikimedia.

The structure of a Dengue virus consists of a core, an envelope, and proteins attached to it. The core contains the genetic information of the Dengue virus. This genetic information codes for the production of a new virus as well as the creation of a new core, envelope, and envelope proteins. The viral envelope can fuse with the outside of a human cell, and also allows for the attachment of proteins. The envelope proteins are used to attach the virus to the outside layer of a human cell.

 

A study done by Karen Clyde sheds light on how the virus uses a replication cycle that involves entering a healthy human cell and hijacking numerous cell processes to complete replication. First the virus attaches to a human cell using proteins on the outside. Then the human cell brings the virus into its body, resulting in the release of the virus inside the cell. The next step involves the formation of a structure for reproducing the virus using materials found in the human cell. Finally, this produces a chain of viral proteins which are broken apart to form the final product of a core surrounded by an envelope with proteins.

 

In my opinion, travelling through a foreign country and having to be constantly aware of mosquitoes is annoying, but well worth the effort. Next time you travel through a country at risk of Dengue infection, make sure to coat yourself in bug repellent. As there are no known vaccines or treatments for Dengue, once infected, it will accompany you for the rest of your life. Therefore it is up to you to take appropriate precautionary measures to protect yourself.

Menopause: Why Do Humans Experience It?

It has always been a mystery as to why humans go through menopause. Chimpanzees and bonobos, whom we share 99% of our DNA with, are still able to reproduce throughout their entire lifespans while humans in their latter part of their lives become infertile. What then, are the reasons for humans becoming infertile as they get older?

Recently BBC and the Atlantic has published an article that can potentially explain the cause of menopause. Menopause is actually a unique trait shared by only three species: humans, killer whales, and pilot whales. Scientists have made many predictions as to why humans may experience menopause, but these predictions end up being extremely difficult to test. One possible explanation for menopause is the Grandmother Hypothesis. The Grandmother Hypothesis suggests that humans have given up their reproductive potential to focus more on caring for their children and grandchildren. In order to prove this hypothesis, we must prove that children are more likely to survive when their grandmother is present compared to when she isn’t. Dr. Lummaa from the University of Turku has published a study that shows that children are 12% more likely to survive adulthood if their grandmother is present.

Elderly Woman Image by Kawahara via Flickr

With the help of advanced technology, humans nowadays are able to live longer than ever, and women often outlive the lifespan of the female reproductive system. However scientists are still finding it. This hypothesis is near impossible to test because humans’ current fertility rate patterns are different from the fertility patterns of our ancestors.

Surprisingly, if we investigated other species that also experience menopause, we could possibly relate this information back to the human species. Darren P. Croft, an animal behaviorist at the University of Exeter, used years of data to reveal why killer whales potentially experience menopause. It became clear that from an evolutionary standpoint, it is a disadvantage for the mother and daughter killer whales to be impregnated at the same time. When both the mother and daughter killer whales reproduce during the same period, the newborns of the older generation have a higher chance of dying, approximately 1.7 times more likely as opposed to the younger generation. According to Croft, “this new research shows that old females go through the menopause because they lose out in reproductive competitive with their own daughters.” This research on killer whales provide us with an explanation for menopause that may possibly apply to humans.

Killer Whales: One of the three species that goes through menopause
image by Grit via Flickr

All in all, it remains a mystery as to why humans go through menopause. Although there are many existing hypotheses that may potentially explain menopause, nothing has been scientifically proven. However, we can gain insight into why humans experience menopause through observing other species.

-Andrew Ting

The Zika Virus: Are we done with it yet?

Zika virus transmission. Imagine courtesy of World Health Organization (WHO).

In April 2015, an epidemic of the Zika virus disease took Brazil by storm , quickly spreading through South America and parts of North America. The disease was relatively unknown but it was associated with causing microcephaly, a brain defect where a baby’s head is significantly smaller than normal in fetuses of pregnant women with the Zika virus. As time passed with nearing proximity to the 2016 Summer Olympics in Rio de Janeiro, the possibility of contracting the disease caused so much concern that many athletes withdrew from competition.

But the Zika virus is not new.

It was first discovered in 1947 in monkeys of the Zika Forest in Uganda. Since then, there have been small outbreaks in the equatorial Africa and Asia. Unfortunately, due to the mild symptoms of the disease and it’s similarities to dengue, yellow fever and chikungunya, it was often overlooked and underreported until its recent outbreak.

The virus is mainly spread by mosquitoes of the Aedes genus common, namely A. aegypti and A. albopictus. Transmission to a fetus during pregnancy increases the risk of many birth defects such as microcephaly in the newborn child. If that doesn’t sound scary enough, it can also be spread from sexual activity and blood transfusions!

Microcephaly in newborn infants as a result of the Zika virus. Image courtesy of the Boston’s Children’s Hospital.

So what are the symptoms? Infections from the Zika virus will only display mild symptoms at most, including fever, rash, joint pain, pinkeye (conjunctivitis), muscle pain and headache. Symptoms typically subside after a week. Travellers and pregnant women who have recently visited affected areas should be tested for the disease.

The Zika virus is no longer an epidemic by the World Health Organization (WHO) as of November 2016 and it seems like something of the past. But why should we still be concerned about it?

Sadly, just because it is no longer an epidemic doesn’t mean that you shouldn’t be worried. Right now there is no cure for the Zika virus. Vaccines are being developed as we speak, but they probably won’t be available for another 10 years. For now all we can do is take precautions against mosquito bites and avoid travelling to places affected by Zika, especially if you are pregnant.

The problems Zika poses are far from solved. The Zika virus outbreak highlights the just one of the detrimental effects that poor sanitation has on our population. If we don’t solve the problem of managing mosquito populations and inventing new vaccines, we will have persisting human vulnerabilities to mosquito-spread diseases. We will always be at risk of infection to a disease we haven’t cured.

In the meantime, be mindful of your travel plans and check out this video on the Zika virus!

  • Florence Ng

Dengue Fever Virus: What Is it?

One day, while you are on a tour through India, you notice that mosquitoes have bitten you several times while you were unaware. You disregard it, thinking that these bites have never amounted to anything more than an annoying itch on a bump in the past. However, several months after returning home, you begin to feel fatigued, nauseous, as well as sporting a severe headache. At this point, you may have been infected with Dengue fever virus.

General cross-section view of a Dengue virus particle. From Wikimedia Commons.

According to an article published by Anne Gubler in Trends in Microbiology, Dengue fever virus originated from the tropical regions of the Earth during the 18th to 19th centuries. The virus is mainly spread through a species of mosquito referred to as Aedes aegypti, which still populate the tropical and some subtropical regions today. However, as human population continues to rise in these areas, the risk for another epidemic outbreak further increases. This is due to the ease at which a mosquito can travel from host to host, spreading the virus at a much higher speed.

General cross-section view of a Dengue virus particle. From Wikimedia Commons.

The structure of a Dengue viral particle consists of a core, an envelope, and proteins attached to it. The core contains the genetic information of the Dengue virus. This genetic information codes for the eventual production of a new viral genetic strand, as well as the creation of a new core, envelope, and envelope proteins. The viral envelope can fuse with a host cell membrane, and also allows for the attachment of proteins. The envelope proteins are used to attach the virus to the receptors located on the outside membrane of a host cell.

A study done by Karen Clyde sheds light on how the virus uses a replication cycle that involves entering a healthy host cell and hijacking numerous cell mechanisms to complete replication. Firstly, the virus binds to a host cell using its envelope proteins. Secondly, the host cell internalizes the virus, resulting in the release of the viral package inside the cell. The next step involves the generation of a viral replication enzyme using host cell structures before the virus genome can be replicated. This eventually results in the production of a chain of viral proteins, which are cleaved apart to form the final product of a core surrounded by an envelope with proteins.

Next time you travel through a country at risk of Dengue infection, make sure to coat yourself in bug repellant. As there are no known vaccines or treatments for Dengue, once infected, it will accompany you for the rest of your life. Therefore it is up to you to take appropriate precautionary measures to protect yourself.

-Angus Lee

Fatal Fentanyl: The Pill that Kills

Imagine this house party scene: I’m stuck at my best friend’s house for his birthday, and everyone is either passed out or wasted. My best friend comes up to me, insisting that I try fentanyl for the first time.

“Just one pill. It won’t hurt,” he says persuasively, and places the green pill on my palm.

Fentanyl is often crushed into powder using a pedicure file and then snorted for rapid ingestion. Image Credit: CalgaryPolice.ca

Peer pressure  gets the best of me, and I reluctantly swallow the green pill. “It can’t be that bad, could it?” I question myself.
As it turns out, swallowing that fentanyl pill was one heck of a big mistake!

Image illustrating that less than a dime-sized amount of fentanyl can fatally harm a person. Image Credit: abbypd.ca

A single green pill contains merely 50mg of fentanyl, which was more lethal than I thought! A deadly dose of fentanyl is just 2mg, which is equivalent to 2 grains of salt. Fentanyl works by binding to opioid receptors in the brain, which control a person’s breathing rate, emotions, and pain. This produces a state of peaceful happiness for the user, making fentanyl a highly addictive drug. However, fentanyl can stop a person’s breathing as a side effect, which can lead to death. Fentanyl is an opioid drug that is up to 100 times stronger than morphine! The high potency in a small amount of fentanyl misleads users to take a higher dosage, and greatly increases overdose risk.

Recreational drug use is on the rise, and many street drugs are unknowingly laced with fentanyl. Fentanyl’s potency makes it attractive for drug traffickers to smuggle many doses into a small package, but proves to be deadly for users.

Barcharts illustrating the number of deaths caused by fentanyl overdosage in BC. The number of deaths 2015 was ten times more than the number of deaths in 2012.
Image Credit: cbc.ca

B.C’s drug overdose crisis continues to take a terrible toll, and most are fentanyl-related deaths. Since July 2016, every 4/5 drug testings in Vancouver were positive for fentanyl. Teenagers and young adults aged 16 to 21 are the most vulnerable to drug abuse because of their high tendency for experimentation.

An important tool to help fight against B.C’s fentanyl crisis is the Naloxone kit, which contains medication that reverses the effects of opioid overdose. BC‘s Take Home Naloxone (THN) Program provides training and resources for overdose emergencies. Naloxone is now available to the public without prescription. It is easy to use, has small side effects, and provides a life-saving solution! Increasing access to naloxone is the fastest way to reduce the dramatic number of fentanyl-related deaths.

BC’s Naloxone Program distributes lifesaving kits and trains people to use overdose-reversal medication. This video shows how to recognize and respond to an overdose. Video Credit: towardtheheart.com/

Poster outlining the dangers of drugs cut with fentanyl. Image Credit: knowyoursource.ca

As I mentioned before, fentanyl binds to opioid receptors, which puts the person in a state of ‘suffocating happiness’. Naloxone works by knocking out fentanyl from opiate receptors in the brain and restores normal breathing rate. Without Naloxone, brain cells die without proper oxygen circulation, and the drug user receives serious brain damage and memory loss.

If you decide to take recreational drugs, be sure to carry a Naloxone kit with you. In addition, don`t be afraid to call 911 if you sense that someone is overdosing. Don’t let either you, or your friend, become another fentanyl victim.

-Anna Tam

 

Brain is Smoking Tobacco.

“Let’s smoke, I am stressed out” may be what you have said, but you had smoked two packs for the past 15 hours.  This might describe a life of some university students. Through education and experience, you have learned that the substance abuse certainly leads to bad endings in every way. Low GPA is not only the result that would stir every student on a personal level, but also it is a destructive disorder that ruins your health, your social life, and your brain function.

Drug addiction is a chronic disorder that involves the compulsive drug-seeking and -taking behaviors. Now, you are determined to quit because it is 2017 now, and all your friends are stopping. Soon all your friends have quit, but your addiction even worsens because drug withdrawal strengthens your dependence. You wonder why because you have used same methods.

Addiction has many variables: personal and biological elements. Various reasons such as pleasure, stress, peer pressure , or curiosity may have started this behavior. It might be a matter of morale or willpower. Later, an addiction becomes a mental problem because an addiction hard-wires our brain to yearn and take more and more. Addiction involves modifying our brain chemistry. Brain is a center of our consciousness and it is composed of interconnected network of neuronal cells. The cells process and relay signals so we can respond to the environmental stimuli. A reward system in brain chiefly incorporates the release of the neurotransmitter (NT) called dopamine.

TED talk by Nora, Volkow, talk on effect of addiction on brain

Dopamine is a messenger chemical that infuses motivation and directs a set of behaviors towards a rewarding experience. The cells would carry a motivation signal as electrical signal by receptor-NT relationship. One cell receives NT by its specific receptor and carries an electrical signal to other cell, releasing a NT. Nicotine, bona-fide  psychoactive agent, reinforces the reward system leading to addiction. A sustained tobacco use desensitizes the effect of chemical in the brain, hence weakening the a self- regulation. Mental disorder has a deep link with the genetics, not only with the environment. Since biology is dictated by the DNA, there would be some credibility to study the effect of gene in smoking behavior pattern. According to 2003 review article in reputable NEJM (New England Journal of Medicine), Drs. Jordi Cami and Magi Farre have pointed out that nicotine dependence is linked to one version of dopamine receptor gene, named to be TaqIA D2.

In conclusion, a chemical influences our behavior because a brain is composed of cells. We have different susceptibility to this chemical cue because we all carry different DNA, even identical twins!!

-Jiwon Kim-

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All Aboard the Hype Train!

Recall the most recent technological or scientific breakthrough that you were hyped about – a new Apple product, SpaceX, coffee mugs that charge your phone, or one of the other innumerable findings in the realm of science. How long did the excitement last before – and after- the slated release of the technology you have in mind? When it finally arrived were you satisfied, let down, or left somewhere in between?

In the field of genetics, a new approach which utilizes the CRISPR (pronounced as crisper) mechanism to edit the genomic DNA of a target host holds great potential and is seen as the next “big thing”. CRISPR and its associated genes (cas) are part of an immune system used by prokaryotes to identify and degrade foreign DNA. Geneticists have modified the CRISPR/cas system to help them accomplish amazing feats. Compared to previous methods of genetic modification, CRISPR/cas is far more streamlined and efficient.

The CRISPR/cas mechanism has seen numerous applications in fields ranging from botany, pathology, and even oncology. CRISPR/cas has also proven to be able to restore the non-functional gene which leads to sickle cell anemia.

The hype behind this emerging technology should be kept in check, however, as CRISPR/cas is not a magic bullet. Though its potential seems limitless, it does have technical limitations. Furthermore, an international consortium on stem cells, ethics, and law known as The Hinxton Group has released a statement warning about the potential dangers of unfettered application of CRISPR/cas to biomedical research.

We know that the media and the scientific community both love to get excited over new and upcoming technologies, and the hype for CRISPR is no different. As potential consumers and beneficiaries of CRISPR/cas mediated treatments, we like to hear about the wonders of this emergent technology. However, as scientists and communicators, we ultimately have to be careful that we do not lead ourselves and our target audience through the hype cycle. Over promising and underperforming is a hallmark of bad science, and is something we should strive to avoid.

Something Is Still Active After Death –“Zombie Genes”

In a recent study, scientists have discovered that over 1,000 genes were still active in mice and zebrafish after their death. Does this mean they will turn into the “zombies” we see in the movies? Not quite, but it shows that dead bodies might be more alive than we previously think.

Are we really “dead” after we die? Source: http://www.publicdomainpictures.net

What is death? We normally consider someone or something to be dead when they stop breathing and their hearts stop beating; all the cells in the body will stop functioning and eventually decay. However, this new discovery showed that some genes will only become active after the host “dies”. This makes us think twice about the definition of death.

“The headline of this study is that we can probably get a lot of information about life by studying death,” said Noble.

Peter Noble, the leading scientist of this study, and his colleagues were surprised to find there was a lag period before these “zombie genes” became active; most of the genes became active 30 minutes after death, and some up to 48 hours after death. They also found that some of the genes remained active for up to four days. This reminded me of how a corpse turns into a zombie in a similar fashion.

 “What’s jaw-dropping is that developmental genes are turned on after death,” said microbiologist Peter Noble.

The functions of these zombie genes shocked the scientists the most. They found developmental genes, which are responsible for embryo formation, were activated after the animals died. This means that scientists can potentially extract these zombie genes and create a new living cell – in other words, reanimating the dead.

Active developmental gene were found in dead organisms. Source: pixabay.com

The scientists found cancer-promoting genes were also part of these zombie genes. This gives potential explanation for why patients who received organ transplants have an increased rated of developing cancer. As a potential medical application, we can make sure patients do not develop cancer from transplants by measuring the zombie gene activity before the surgery.

In another study, scientists suggest zombie genes can also potentially help forensic scientists narrow down the estimated time of death more accurately. This provides crime investigators an additional resource to help them solve cases.

Although this study gives a glimpse hope that zombies might one day exist, there are still many factors such as decomposition to consider. The “living dead” would need to fight though trillions of microbes and maggots to prevent decomposing. Below is YouTube video by AsapSCIENCE explaining what happens to our body when we die.

While the belief of zombies and reanimating the dead are still a long way from becoming reality, a living corpse is very much real.

By: Kevin Chao