Category Archives: Portfolio

Learning Journal #5

1.

The portfolio I will be viewing is written by Campbell Drohan.

2.

Based on Campbell’s BIOL 463 portfolio, I would say that her biggest strengths are reflectiveness and critical thinking. Campbell was very reflective of her learning, which is particularly evident in her learning journal #1, techniques café reflection, and final project reflection. In these posts, she connected new knowledge with what she already knew and was cognizant of the learning objectives that she met through each assignment. In addition to being reflective, Campbell demonstrated great critical thinking in her post titled, “Appreciating the negative in scientific research,” in which she provided a thoughtful argument of how publishing negative results in scientific papers can prevent other researchers from pursuing projects that have already been done.

3.

I believe that one thing Campbell learned, that I did not, from the final project is a method to assess tumourigenicity using xenografts. One thing that I learned, that I think Campbell did not learn, from the final project is a method to extract alveolar macrophages through bronchoalveolar lavage.

As mentioned in my previously posted reflection to my final project, the most valuable lesson I learned from doing my final project is to be persistent when facing a challenge. There were often times when I had difficulty expressing my ideas in writing and it felt frustrating to be at a standstill when I wanted to move forward with my project. For example, the background section of my project was challenging for me to write because I had consulted many previous studies that served as the inspirations my research question, but it was difficult for me to figure out how to integrate these previous studies in my background section in order to set the stage for my project. Despite the difficulty I experienced, I kept working at it and I was eventually happy with what I wrote. Though the challenges of the final project for BIOL 463 are over, there will still be plenty of challenges to face as I move forward, and I believe that persistence will help me overcome them.

Final Project: Annotated bibliography

Here is the link to the annotated bibliography of my final project: annotated-bibliography

(Note: An annotated bibliography was included in my project outline located in a previous blog post, but it has been updated since then. The version posted in this blog post is the updated version.)

In my annotated bibliography, I commented on 10 scholarly works that I consulted the most while working on my final project. Most of the articles were used to familiarize myself with what is known in the field in order to find a gap in the literature that my project could potentially address, while other articles were used to devise an experimental approach that would lead to findings that would fill that gap. Creating an annotated bibliography during the planning stages of my project was helpful because by writing a short summary of what I considered to be the most relevant information from each article, I did not have to sift through large amounts of text more than once. Although a lot of time was put into making the annotated bibliography, a lot of time was also saved by not having to read whole journal articles over and over again.

Final Project and Layperson Summary

Here are the links to my final project and its layperson summary, respectively:

leon-lai-final-project

leon-lai-layperson-summary

Looking back at the experience of doing my final project, I would compare the experience to hiking the Grouse Grind. Both involve a long and arduous journey that is constantly challenging, and the finish line can often seem far and out of reach. But with hard work and determination, what seem to be insurmountable challenges can be overcome. And when the journey is finally complete, you gain a great sense of accomplishment.

One of the biggest lessons that I will take away from the final project is to be persistent when facing a challenge. There were often times when I had difficulty expressing my ideas in writing and it felt frustrating to be at a standstill when I wanted to move forward with my project. For example, the background section of my project was challenging for me to write because I had consulted many previous studies that served as the inspirations my research question, but it was difficult for me to figure out how to integrate these previous studies in my background section in order to set the stage for my project. Despite the difficulty I experienced, I kept working at it and I was eventually happy with what I wrote.

Though the challenges of the final project for BIOL 463 are over, there will still be plenty of challenges to face as I move forward, and I believe that persistence will help me overcome them.

Top Three Assignments: Loh et al. (2015) Assignment

Here is the link to my Loh et al. (2015) assignment: loh_et_al_assignment

I consider the Loh et al. (2015) assignment to be one of my top assignments because I was able to answer the questions more confidently than previous assignments. Leading up to this final in-class assignment, I had received lots of practice reading journal articles for previous assignments, which helped me read the Loh et al. (2015) article more efficiently. As a result, I was confidently able to interpret the results and figures of the paper. Overall, this assignment made me realize how much I have grown in terms of my ability to read and extract information from primary research articles, which will help me in future courses.

Top Three Assignments: Chiesa et al. (2012) Assignment

Here is the link to my group’s Chiesa et al. (2012) assignment, with additional answers to questions that were not answered previously and modified answers to address comments: chiesa_assignment_2016

I chose the Chiesa et al. (2012) assignment as one of my top assignments because it demonstrated how I was able to draw from concepts that I learned in earlier sections of the course and used them to hypothesize mechanisms that contribute to disease phenotypes. More specifically, I recognized that epigenetic regulation strongly contributes to abnormal gene expression patterns associated with Beckwith-Wiedemann syndrome.  In addition, I considered this assignment to be engaging because it involved a paper that studied humans. Many of the papers covered in this course used animal models, so it was nice to look at humans through a clinical study for a change.

Intermediate Stage of Project: Project Outline

Here is the link to my project outline: project-outline

One lesson that I learned while working on my project outline was that dividing the work over the span of many days was an effective approach to completing a large assignment. My strategy to complete the project outline involved setting intermediate deadlines for each section so that when the deadline for the full outline approached, I was not overwhelmed with a lot of work. This strategy worked well for me and I plan to use this strategy again for future projects in other courses.

Top Three Assignments: Group 3 Honeybee Assignment

The assignment for the honeybee unit was one of my favourite assignments because it provided the opportunity for different groups in the class to propose different mechanisms in a process of interest, and eventually combining all of the information into a single model. I enjoyed both sharing my group’s paper and learning about the papers assigned to other groups. I found it fascinating that even though each group presented a different mechanism that influenced caste switching, none of the ideas contradicted each other, but instead, complimented each other when combined into a single, consistent model.

Through this experience, I learned that there may not be a single and simple answer to a research question. As demonstrated in the honeybee unit, there can be multiple mechanisms involved in a process, which highlights the importance of having an open mind to different points of view. Overall, this assignment showed me that collaborating with others who have different ways of thinking is an effective way to achieve a fuller picture.

Here are the links to the questions assigned to my group and my answers, respectively:

group3_paper_and_questions

answers-to-group-3-questions

Unprompted post: Haiku about genetics

In the form of a haiku, I describe how I view the study of genetics:

Genetic puzzle.

Small pieces of big picture

Help find the answers.

To elaborate, I consider genetics to be like a puzzle because it is a mystery that has yet to be fully solved. In my opinion, there is much that we can learn from studying genetics because it is connected to all life sciences. I believe that the more that we learn about genetics, the more that we can understand the inner workings of all living things, including ourselves.

Unprompted post: Midterm #2 Reflection

Midterm #2 was a learning experience for me. Beyond getting to know an academic paper in great depth, one of the biggest lessons that midterm #2 taught me was that I should be more skeptical of what journal articles claim. This was especially clear to me when we did the group component of midterm #2. Through collaborative discussion, my group came to the consensus that the paper for midterm #2 presented data that showed a correlation between peculiarities of the Xi in female lymphocytes and predisposition of expression of normally silenced genes, but the findings presented were not sufficient to show a cause and effect relationship. In order to confidently establish a cause and effect relationship, the authors needed to perform a manipulative experiment to induce “the cause” (i.e. changing Xist RNA cloud localization patterns) to see if we can observe the expected effect (i.e. changes in gene expression). However, instead of performing a manipulative experiment, the authors performed an observational study, from which they could only observe correlations.

In the past, whenever I read journal articles, I always accepted the claims that researchers made. But from now on, I will be more skeptical of claims and think critically before I accept them.

Response to prompt: Public opinion of genetically modified foods

Prompt:

Genetically engineered foods are greatly criticized, and often feared, by many people. Initially people feared that exogenous DNA from the food item could then transfer to the consumer’s cells and cause harmful effects. More recently (and with the advent of siRNA technologies) there is a worry that ‘transgenic RNA’ from the food could get into the consumer’s cells and cause misregulation of the consumer’s genes. What is your view on this, and how would you present it to someone who has no biology background?

As an informed genetics student, I do not share the fear that some people have against genetically modified foods. In fact, I support genetic modification of foods because there are several benefits such as increased yield and nutrition, and decreased need for the use of pesticides.

However, I can imagine that many people who worry about the consequences of genetically modifying food may not have a background in the study of genetics, and are thus uninformed about what genetically modified foods can and cannot do. Therefore, by educating the public about genetics, we will be able to reduce misconceptions that may contribute to the public’s concern about genetically modified foods.

One way to better inform the public about genetics is to incorporate more genetics at the high school level in general science courses. Personally, I did not learn very much about genetics until I started studying in university and enrolled in genetics courses. Therefore, I would expect most people to have a limited knowledge of genetics unless they decided to pursue the study of genetics at the post-secondary level. If more genetics were taught at an earlier age, when general science courses are mandatory, I believe that the general public would be able to make more informed opinions regarding genetically modified foods.