Global Warming: Victims to our Ignorance

Global Warming and the Arctic region

Everyone has at least once, in their lifetimes, have heard of the term “global warming,” especially nowadays, when it has become a major issue. Anthropogenic activities are the largest contributors to the increment in global temperature. On average, the Earth’s surface temperature has increased by 0.6℃ since the 19th century and is to further increase. This increase has a critical impact in the Arctic, where glaciers are receding and sea ice is disappearing. The CBC News has announced that the Arctic ice would vanish in 10 years; thus, primarily affecting the Arctic ecosystems.

An analysis from the CryoSat-2 satellite indicates that 900 cubic kilometres of Arctic ice has disappeared each year since 2004. At such a rate, scientists warn the Arctic could be ice-free in 10 years.

The melting of the Arctic sea ice. Photo by Kathryn Hansen/NASA/Reuters on CBC News

Let’s dive into the Arctic by familiarizing ourselves with the organisms that lives there. Many creatures, from algae to polar bears take shelter in this region. Starting at the microscopic levels of life forms, algae are affected by the continued thinning of the ice. In the Arctic, most of the algae under the ice have died out. Paul Nicklen, a biologist and a photographer at National Geographic, states that “[sea] ice acts like a garden… losing that ice is like losing the soil in a garden.” During the spring, when the sun shines on the ice, the phyto-plankton  start the food chain. On the top of this food chain is the most famous Arctic animal, the polar bear.

The Arctic food chain. Picture by Michael Kline on Dogfoose Magazine Art

How are our activities affecting this circle of life?

Human activities produce pollutants. These pollutants such as carbon dioxide and other greenhouse gases, such as methane cause the increase in global temperature. They collect in the atmosphere like a blanket, causing the heat to trap, warming up of the Earth. The accumulation of these toxic pollutants leads to the loss of the Arctic ice, tremendously affecting the ocean temperatures. Most of the sunlight is reflected back to space by the ice caps, but with the absence in ice, the sunlight will be absorbed by the Earth. Therefore, increasing the ocean’s temperature and resulting in release of more methane, a greenhouse gas, into the atmosphere, further accelerates global warming and raises sea levels. These fast changing habitats are profoundly affecting wildlife.

Can we save the Arctic?

Some warming is inevitable, but we can definitely reduce the rate of it. First, we need to be aware that the Arctic is home to many organisms, ranging from the algae to the polar bears. This is very important because if the Arctic ecosystem fails, we, too, are ultimately affected. The oceans are our life support system. Once we realize that, we can make a difference and save the Arctic and its animals. The Natural Resources Defense Council (NRDC) suggests that we can save the Arctic and our planet by by conserving energy. Changing to fluorescent light bulbs will not only reduce 700 pounds of CO2, but also reduce your energy bill. Also, we can change our cars to hybrid gas-electric engines cut global warming pollution by one-third or more.

photo

Polar bears are dependent on sea ice for survival. Photo by rubyblossom on Flickr

The following video clip shows the seriousness of effects of global warming on polar bears.

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-MinJi Yoo-

 

 

Bacteria Are Mini Factories That Can Produce Fuels !

Have you ever thought about what would happen if you had to go to school or other places by walking or driving bicycle ? We become fearful even by thinking about this situation which happens when we run out of fossil fuels . Clearly, we are all dependent on fossil fuels to live comfortably.

However, it takes many years to produce enough fossil fuels by the decomposing plants.Furthermore, emissions through the combustion of fossil fuels contain harmful substances such as carbon dioxide which lead to global climate change.

Therefore,creating a new renewable energy source without unfavourable emissions is a serious issue to consider. One of the amazing solutions for this problem is achieved by using microbes to make advanced biofuels.

Microbes can produce biofuels.

(adapted from :http://web.mit.edu/press/2012/genetically-modified-organism-can-turn-carbon-dioxide-into-fuel.html)

Scientists in MIT university are trying to use a microbe called Ralstonia eutrophato to make fuel from carbon dioxide. Nitrate and phosphate are important nutrients for this bacterium , but when they are limited, it stores food by forming polymers out of the available carbon. The properties of these polymers are very similar to those of plastics made up of petroleum. By making a few changes to the bacterium’s genetic structure such as adding a new gene, removing a few genes and altering the expression of the other genes, scientists can produce fuel instead of plastic.More interestingly, they are trying to modify the microbe to use any waste product that is a source of carbon to make fuel.

Another advantage of using this microbe is that it automatically releases the isobutanol (a substitution for gasoline) which makes the process easier for the researchers.Fortunately, unlike some of the other biofuels , isobutanol can be used in car engines without any changes.

Up to this point, these scientists have been successful in making genetic changes that result in the production of isobutanol.

According to another study, animal fats and vegetable oils ,such as soybean oils, are commonly used as the raw material to produce biofuels. However, the increasing demand for biofuel production requires us to think of other biofuel sources which are suitable for human consumption. Microbial oils , such as the one discussed above, are better feedstocks for biofuel production rather than vegetable and animal oils due to different reasons : having shorter life cycle, requiring less work and increasing their amounts more easily.

Obviously, teaching microbes to make biofuels is a major breakthrough that is very beneficial and essential for all of the people around the world.

Below you can see a video that shortly explains about producing biofuels with the aid of microbes:

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video posted by “bloomberg”

Maryam Goharian

sources:

“MIT Media Relations”.

http://web.mit.edu/press/2012/genetically-modified-organism-can-turn-carbon-dioxide-into-fuel.html

“Biofuel definition “

http://en.wikipedia.org/wiki/Biofuel

“Global Climate Change” .http://www.cotf.edu/ete/modules/climate/GCclimate1.html

http://apps.webofknowledge.com.ezproxy.library.ubc.ca/full_record.do?page=1&qid=6&log_event=no&viewType=fullRecord&SID=2FnGmdEIaFKiJ3Mn25A&product=UA&doc=1&search_mode=GeneralSearch

https://www.youtube.com/watch?v=zBfjKYM9fLM

 

Picture from:

https://blogs.ubc.ca/communicatingscience2012w109/files/2012/09/biol200-image.jpg

Creatine: Quantity Over Quality

          Creatine has become a very popular and sports supplement today and is used by many athletes to improve their performance in high-intensity sports because its energy-inducing effects are undeniable, but the supplementation is highly dependent on a very high volume of ingestion to actually render these effects.  As a result, popular supplement companies tend to include this name in products to raise the price, while in reality, many of them only include minimal amounts into the actual product.

Creatine is a molecule that is produced in the body that aids in providing energy for the cells through the mechanism for ATP synthesis by phosphorylation. Creatines’ role is carried out by a special kinase. This function is depicted by the following link:

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-creatine involvement in ATP synthesis (by ctacac)

By supplementing diet with extra creatine, the idea is that there will be higher abundance of fuel for ATP synthesis which in turn will project a higher performance in high-energy demanding actions, Dr. Rutledge discusses these effects to a broad audience in the following interview:

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Creatine discussion on nutrition (Dr. Rutledge)

The downside to this supplementation however comes down to the practice of how it must be taken. The most basic and trusted form of the compound is called creatine monohydrate. This compound readily forms an acidic bi-product called creatinine under the acidic conditions of the stomach. Thus only a fraction of the original desired molecule is actually absorbed. In effect, the only way to actually saturate the muscles with creatine is to ingest about 20g per day for about a week to compensate the loss to formation of the bi product. The acidic state of the stomach and its effect on creatine can be seen from the following illustration:

Creatine vs pH

Creatine conversion to creatinine under pH (http://www.integratedsupplements.com/email/images/CreatineGraph.jpg) Howard et al.

 

Here we see that over time, ingested creatine monohydrate will indeed degrade, which in turn hinders its usefulness.

The dirty play by popular pre-workout brands is that they exploit the name of creatine to jack up prices, while most pre-workout products only contain 5-10g, hardly an effective dose for a single workout. One such example is given by ‘SuperPump Max’ by Gaspari Nutrition, where creatine is noted in their ‘propriety blend’:

SuperPump label

SuperPump label (~11.3 grams of creatine) (http://www.nutritionwarehouse.com.au/upload/image/supering%281%29.jpg)
Gaspari Nutrition

This product is sold at about $50 for around 600g, which is quite expensive considering such a large serving size(16g). If the word on the street is that creatine should be loaded at 20g for about a week to reach effective levels, then what use is the small portion included in SuperPump other than to boost marketing and price?

Since this effective supplement may only be effective in high doses, the quality of products sold by major brands is very questionable when high prices are compared to the actual product. Thus the low potency of creatine monohydrate makes this supplement a case of pure quantity in that the desired effects of the product can only be achieved through a high intake over time. Clearly, is impossible to achieve through many pre-workout supplements that boast creatine in their labels, but fail to include adequate amounts.

-Surbinder Bolina

 

Changes to the kidney transplant waitlist

Kidneys are the body’s primary mechanism for blood filtration and waste removal.  Kidney failure leaves individuals with two options, a kidney transplant or dialysis.  Dialysis does filter the blood, but it does not fully replace kidney function.  This causes wastes to gradually accumulate and leaves patients with numerous painful side effects.  Therefore, kidney transplants are the only way to successfully return kidney function to normal.

 

This image shows a dialysis machine and is from Bryanking.net/dialysis

 

Unlike Canada, the United States and most of Europe has centralized national waitlists for kidney transplants, which take age and health into account.  Kidney transplant waitlists in Canada are dependent on which province an individual is in, and within the province they are further divided by region.

The nationalization of transplant waitlists would prevent the unequal wait times we see between provinces in Canada. For example, British Columbians are forced to wait three times as long as people from Nova Scotia or Quebec. B.C. residents’ wait-time is roughly 2145 days, where as Nova Scotia residents’ is 765 days and the national average is 1258 days.  The video below is a CTV news interview and it describes one individual’s life with kidney failure and Ontario’s transplant wait times.

This video is uploaded by LHSCCanada and is a CTV News interview

The Canadian government needs to reassess the way donated kidneys are distributed.  In the United States, the government committee that oversees kidney transplants is taking initiatives to make better use of the countries donated organs.   They have decided to push forward with distributing donated kidneys based on an individuals estimated age and health.  This would give younger individuals healthier and younger kidneys.  Despite criticism, this scheme has the ability to decrease the number of returning recipients to the waitlists.  On the contrary, Canadians’ ability to receive a transplant depends on where an individual is and how many people are competing for an organ.

This image essentially shows what happens with a kidney transplant and is from Medline Plus/kidney transplantation

 

Canada is in dire need of updating its waitlist guidelines.  From family experiences, I know the consequences of Canada’s regionally divided organ waitlists.  Dialysis does provide a temporary solution removing and filtering blood, but it is not permanent.  People’s lives become centered on their dialysis treatments, which occur 3 times a week.  Individuals’ with kidney failure are forced to endure pain and sickness as toxins and wastes accumulate and lead to frequent hospital visits.

 

-Ryan Sidhu

The Dark Side of Video Games

Imagine yourself playing a video game that was so enthralling, that you could not put away the controller, and after you finished you’ve realized that your entire day has passed by. Then you continue this behaviour the next day and so on and so forth. This is the dark side of gaming.

Video game addict from http://www.evilcontrollers.com

Playing too much video games can be extremely problematic and can even lead to addiction. The main issue that results from playing too much video games is the impact on life skills.

Are video game addictions real? Are they to be taken seriously? Watch these videos of Dr. Marc Kern (Addiction Expert, Director of Addiction Alternatives) examining the aspects this relatively new problem in human society. The first is simply on defining what a video game addiction is, the second is on why video games are so addicting, and the third is on explains the common effects of this addiction. (I found these videos all together in video, however the blogging program would not allow me to embed it)

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The consequence of playing too much video games are examined by a study by Charlotta Hellström, at Uppsala University in Sweden, examined 7757 Swedish adolescents found that the found most common gaming-related consequence was getting less sleep.1 As we all know, getting less sleep can severely impact productively the next day. The second most common consequence from gaming was not having enough time to do school assignments.1 This consequence is probably the most significant to us university students. The other consequences that had arisen from gaming include, increased conflict with parents and/or siblings, not having time to spend with friends, and not having enough time or forgetting to eat.1 Although, video game addictions are not as life-threatening as other addictions, it does not mean that it should not be taken seriously. Video games are fun, and can be a good thing. However, too much of a good thing is bad for you. A common saying that should be applied to video games. Most things in life are best enjoyed in moderation.

References

1. Hellstrom, C., Nilsson, K. W., Leppert, J., & Aslund, C. (2012). Influences of motives to play and time spent gaming on the negative consequences of adolescent online computer gaming. Computers in Human Behavior, 28(4), 1379-1387. doi: 10.1016/j.chb.2012.02.023

BPA: Can we limit our exposure?

Here we are, yet again, discussing BPA and the effects of exposure to the nasty chemical. BPA (bisphenol A) is a chemical which mimics estrogen in the human body. It has been linked to cancer, cardiovascular disease, sterility, diabetes and recently: obesity (Wikipedia, 2012). Not only has the chemical been found to be harmful when ingested, it has also been discovered to have a high dermal absorption coefficient; in other words, it is easily absorbed by the skin. So why is it still being ingested by millions of people and estimated to be present in 93% of us? (Prosolia, 2012)

The Canadian government was the first to recognize BPA as a toxin in 2010 and the United States’ FDA finally followed suit this year and banned the use of the chemical in baby bottles and sippy cups (The New York Times, 2012). Unfortunately, BPA is still being used today as a lining for aluminum cans (Science Daily, 2012). Many companies have recently added the tag ‘BPA free’ to their products to keep consumers buying, but are we really aware of the amount of BPA we ingest daily and how to prevent it? Did you know that the new plastic Canadian bills have the highest BPA levels measured in comparison to 9 other countries’ currencies? Research suggests the BPA content in thermal paper rolls, such as those used to print receipts in retail stores, can be passed onto bills simply by contact (Prosolia, 2012).

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The epidemic of obesity in children and adolescents, and its potential causes, has been widely talked about but it has not yet been tied to a specific environmental chemical. A recent study has made history by being the first to use a nation-wide sample of children and adolescents to observe a correlation, if any, of BPA with obesity. The sample size was 3000 children and adolescents between the ages of 6 and 19. The participants were randomly selected to have urine analysis of BPA concentration in order to find any correlation between their BPA levels and obesity. The significant finding: white children and adolescents with high BPA concentrations in their urine were 2.6 times more likely to be obese over those with low BPA concentrations. According to researchers, these results are significant enough to show a correlation between BPA levels and obesity, but the direct process of how the chemical may affect obesity, if in fact it does, is not known.  The study was well controlled, making sure to account for everything from caloric intake to the chance of other phenols, such as soaps and sunscreens, being the cause for correlation. The researchers suggest that the best way to limit BPA exposure in children would be to eliminate it from aluminum cans (Science Daily, 2012).

http://www.calgaryherald.com/life/Study+finds+connection+between+cans+bottles+childhood+obesity/7259888/story.html (accessed September 18, 2012)

Whether BPA attributes to obesity or not, there has been enough research done to show its harmful effects. Why isn’t it banned from all products used in food production? Or banned completely due to its ability to be absorbed so easily by the skin? I am proud of Canada for being the first nation to recognize BPA as a toxin and to eliminate it from baby products, but why is it still being fed to children, adolescents and adults? So much more needs to be done.

-Kady McCappin

Sources:

Science Daily. “Higher Levels of BPA in Children and Teens Significantly Associated With Obesity.” http://www.sciencedaily.com/releases/2012/09/120918111125.htm (accessed September 18, 2012)

The New York Times. “F.D.A. Makes It Official: BPA Can’t Be Used in Baby Bottles and Cups.” http://www.nytimes.com/2012/07/18/science/fda-bans-bpa-from-baby-bottles-and-sippy-cups.html?_r=0 (accessed September 18, 2012)

Wikipedia. “Bisphenol A.” http://en.wikipedia.org/wiki/Bisphenol_A#Canada (accessed September 18, 2012)

Youtube. “Uncovering Bisphenol A – Covert Depopulation.” https://www.youtube.com/watch?v=tFPXxSmLZgE&feature=related (accessed September 18, 2012)

Prosolia. BPA In Currencies:

  1. Chunyang Liao and Kurunthachalam Kannan, High Levels of Bisphenol A in Paper Currencies from Several Countries, and Implications for Dermal Exposure , 2011, Environ. Sci. Technol. In press.
  2. Wolfgang Volkel, Nataly Bittner, and Wolfgang Dekant, Quantation of Bisphenol A and Bisphenol A Glucuronide in biological samples by High Performance Liquid Chromatography-Tandem Mass Spectrometry, Drug metabolism and Disposition, 2005, 33:1748–1757.

The language of DNA

Within each and every living organism, there are millions of books describing things from “how to move” to “how to eat”. These books are written in DNA: language of life. Ultimately, DNA is how nature writes about how to live, and the readers are how all living (and some “dead things”) survive. Even your own body is reading from your own library as you read this blog.

But within this massive collection, we, as people, cannot read most of it because we do not yet understand the language.

However, what we have figured out, thanks to genetics, cellular biology, and biochemistry, are the basic letters of these books; we have the codon table.

Codon Table

The codon table encoding the 20 essential amino acids used in all walks of life. Obtained as a screen capture from http://en.wikipedia.org/wiki/DNA_codon_table

What this codon table tells us is the 20 letters of the DNA alphabet, representing the 20 amino acids. When there are multiple letters together, they can spell a word, also known as a protein.

We have also figured out some parts of these books through areas called open reading frames. These areas use a process called transcription and translation, which is described in the following video found on youtube posted by redandbrownpaperbag.

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We also have clues and insights into some other parts of these books, namely the promoter and termination sequences. These parts tell us where there is an open-reading frame, essentially acting as quotation marks to direct the cell’s attention to a specific location.

Despite all these advances in understanding the language, we know very little in reality.

First of all, there are no known “rules” to writing words, because a word can be as short as 2 letters to as long as a million letters.

Secondly, we do not have a clear picture of how different areas within the genome that are neither open reading frames nor promoters/terminaters interact with the cell, or why those books are there in the first place. These sequences are currently hypothesized as useless, but may be useful as the ENCODE project suggests.

Finally, some words, even with the same spelling, can have many different meanings when they are in different compartments of a cell, so figuring out what they actually mean is quite difficult.

The secret language of DNA might one day be uncovered; someone who can speak “DNA” might be able to take a piece of DNA and then tell us exactly what that DNA is used for, which can advance science from treating and preventing disease, finding new applications for cells (i.e. sustainable fuel), or even figure out what consciousness is. Knowing how to read DNA can open up a brave new world in understanding life as we know it.

Just like how the discovery of the Rosetta Stone lead us to understand the Egyptian language, we may be able to slowly construct our own Rosetta Stone, through genetics and biochemistry, to unravel the mystery of DNA.

The hope is that one day, understanding an organism will be as simple as reading a book.

– Tony Hui

Drug-Free: Treatments for Obstructive Sleep Apnea induced Hypertension

Obstructive sleep apnea (OSA) is a sleeping disorder,and is a highly common problem that occurs during the different cycles of sleep, which has been largely associated with the amount of stress that is exerted on the body. The disorder stems from the recurrent episodes of partial or complete upper airway obstruction during sleep. The vast majority of the people are usually not aware if they have OSA, and these cases are often left untreated for long periods of time, most of which can lead to chronic diseases in the mid 50’s and over the age of 65. OSA has been recognized as the second most popular cause of hypertension, also known as high blood pressure. Hypertension is characterized by the elevation of blood pressure in the arteries, which requires the heart to pump harder and increase the rate of contraction to get blood around the body. The most popular method for treating OSA with hypertension is through pharmaceutical drugs, which includes fluoxetine, tryptophan, and protriptyline; however, these treatments are not as effective as experts say. I argue that drug treatments for OSA and hypertension are ineffective, instead combining multiple treatments such as spine adjustment, physical intervention of nasal airway, and pharyngeal exercises will provide better results in relieving OSA and lowering hyptertension.

YouTube Preview Image This is an overview of OSA. (Krames Patient Education)

For most people who want instant relief from the effects of OSA, over the counter drugs or prescribed drugs are the most common solutions; however, according to studies done by Laurent Stephane from the University of Paris, treatment of OSA and hypertension by antihyptertensive drugs with promising preclinical results have been more difficult and less productive than what the results show. The study composed of  relatively new novel drugs that have entered preclinical trials. However, these drugs are just actually improvements on the previous generations of drugs such as neutral endopeptidase, which acts as an inhibitor for endopeptides. From the extensive tests done on these drugs on rats, there is a low accountability of the drugs producing desired effects. The article even suggests that surgery has been the favored treatment for hypertension, along with the rehabilitation of oral exercises and developing healthier lifestyles.

Fig. 1 This is the structure of aldosterone synthase inhibitor, which is a drug used to treat hypertension. (Antes et al., 2011)

One way of treating OSA is by EPAP (expiratory positive airway pressure) devices; they are placed in each nostril, and have two small valves that remain open on inspiration, but offer resistance to expiration. This device is concocted from extensive studies done on the upper-airway cross-sectional areas by CT scans during respiration. In order to reduce hypertension symptoms, there is another method other than surgery called deep brain stimulation. This treatment is relatively new, however, has promising results. The stimulation on the brain is done by placing electrodes at regions where blood pressure is controlled, and electrical pulses are sent to the brain to inhibit pressor regions. The result is that there is a decrease in the amount of blood needed in certain areas of the body, thus decreasing the amount of contraction the muscles need. Another way of treating OSA is by spinal adjustments, which can be performed by either physiotherapists or chiropractors. Pharyngeal exercises such as singing have shown improvements in OSA patients with hypertension.

Fig. 2 This picture shows how valves on EPAP devices regulate air flow (Doshi et al., 2012)

Fig. 3 The EPAP device is placed in the opening of the nose, and covers the entirety of the nose. ((Kryger et al., 2011)

In this world where everything can be solved by the intake of drugs and antibiotics, the demand of these drugs is always increasing. However, we must ask ourselves, are drugs the only way to solve everything? Through more research and developmental treatments, OSA can be effectively treated by managing a healthier lifestyle along with corrective surgery or EPAP devices, which can reduce the risks and problems of hypertension.

Sources:

Amaro, C. S., Amodeo, C., Bortolotto, L. A., Drager, L. F., Gonzaga, C. C., Paula, K. G., Pedrosa, P. P. &  Sousa, M. G. (2011). The most common secondary cause of   hypertension associated with resistant hypertension. Hypertension, 11(59), 811- 817.

Esler, M., Laurent, S. & Schlaich, M. (2012). New drugs, procedures, and devices for hypertension. Lancet, (380): 591–600.

Video link:

https://www.youtube.com/watch?v=wk8c4rNrQ-A

Websites used:

http://bioinf.mpi-inf.mpg.de/ccb/

http://www.sleepdt.com/provent-nasal-epap-a-skeptic-turns-believer/

http://www.sleepdt.com/a-novel-non-prescription-nasal-epap-device-theravent-to-treat-snoring/

 

Wave Goodbye to Shots: The Future of Drug Administration

A breakthrough by MIT Engineers is making waves in medical drug administration- literally. Previous studies explored using low ultrasound frequency waves rather than a shot or pill to deliver drugs. How is that possible? The ultrasound waves make the skin more permeable allowing drugs to be administered directly on to and passively through the skin. Results of these studies, however, have been lacking.  What researchers have now discovered is the key to success is a combination of both a low and high frequency wave.

Why bother with area of research when we already have pills or shots to give us vaccines or drugs? The possible use of this technique has huge implications on how medicine is produced and administered in the future. Not only is this drug delivery painless, but also direct and noninvasive to the skin.

Watch the video below where Carl Schoellhammer, one of the lead researchers, explains how this technique works:

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Youtube video from user MITNewsOffice

To summarize: a high and low frequency create tiny bubbles in a solution, which implode causing jets of water to remove the very top layer of skin. Drugs are able to be applied via patch or as the fluid the bubbles are created in.

What does this mean for the future?

Image from Google

On a broader scope, if the ultrasound device is made easily available and affordable for wide spread use in hospitals, the need for needles could be hugely reduced. Needle free drug delivery could potentially be more sterile and safer. It could reduce the degree of negative effects or injury from contaminated or damaged needles as well as the dangerous waste of used needles. This change in medical procedure could specifically have a huge effect on third world countries concerning sterility and treatment problems.

Insulin uptake after single freq (blue) and dual freq (red) treatments. Adapted from: Schoellhammer, C. M., et al, 2012

On a smaller scale, practical use of this treatment in the future could changethedaily life and health of diabetics. The two compounds a study used were glucose and insulin, and the results showed that they were successfully delivered passively through the skin.

Eventually, this ultrasound technique could just become an alternative for those who are uncomfortable with swallowing pills or can’t tolerate needles. I, for one, have a vested interest to see where this treatment goes in the future. Because, really, who doesn’t dread getting a shot from the doctors?

– Rachel Chang

References:

Canadian Center for Occupational Health and Safety : http://www.ccohs.ca/oshanswers/diseases/needlestick_injuries.html

Johnson, M.E. et al. “Synergistic effects of chemical enhancers and therapeutic ultrasound on transdermal drug delivery.” J Phram Sci. 12 June. 2012. Web. http://www.ncbi.nlm.nih.gov/pubmed/8818988 (accessed Oct, 1, 2012)

MIT Media Relations. “Getting (drugs) under your skin.” http://web.mit.edu/press/2012/ultrasound-waves-and-drug-delivery.html (accessed Oct, 1, 2012)

Ogura, M. et al. “Low-frequency sonophoresis: Current status and future prospects.” Adv. Drug Deliv. Rev. 3 Aug. 2008. Web. http://www.sciencedirect.com/science/article/pii/S0169409X08000860 (accessed Oct, 1, 2012)

Polat, B.E. et al.”Transport pathways and enhancement mechanisms within localized and non-localized transport regions in skin treated with low-frequency sonophoresis and sodium lauryl sulfate.” J Pharm Sci.10 Aug. 2011. Web. http://www.ncbi.nlm.nih.gov/pubmed/20740667 (accessed Oct,1, 2012)

Schoellhammer, C. M. et al. “Rapid Skin Permeabilization by the Simultaneous Application of Dual-frequency, High-intensity Ultrasound.” J. Control. Release. 22 Aug. 2012. Web. http://dx.doi.org/10.1016/j.jconrel.2012.08.019 (accessed Oct,1 , 2012)

Image source: http://www.europarl.europa.eu/resources/library/images/20081030PHT41044/20081030PHT41044_original.jpg

Schoellhammer, C. M. et al. “Rapid Skin Permeabilization by the Simultaneous Application of Dual-frequency, High-intensity Ultrasound.” J. Control. Release. 22 Aug. 2012. Web. http://dx.doi.org/10.1016/j.jconrel.2012.08.019