Category Archives: Biological Sciences

Organisms Inside Us

Have you ever wondered how many micro organisms are living inside us? Micro organisms are living organisms that cannot be seen with our naked eyes. They may be multicellular (made out of more than one cell) or unicellular (made out of one cell). Our human body is packed with trillions of microbes (or micro organisms). In fact, our own body cells are out numbered with a ratio of 1:10. So, we are technically 90% germs and 10% human. Microbes are found in all parts of our body in different proportions. Our lung has approximately 1000x less microbes than our mouth and has approximately 1 billion times less microbes than our intestines. Although most microbes are harmless to us, some may cause bacterial infections in humans.

The Human Body = 90% Germs + 10% Human

Top three functions microbes in our body:

  1. Defense mechanism: Microbes in our lungs, intestines and our skin provide the first line of defense against harmful bacteria that enters our body. Good microbes found in these areas play an essential role in preventing the spread of harmful microbes by occupying space so less space are available for harmful microbes to settle down inside us. Thus, preventing us from bacterial infections that may cause fever, diarrhea or other problems. Other than that, researchers have found evidence that microbes that live inside us help promote our immune system cells to grow and replicate.
  2. Keeping us in shape: The trillions of microbe colonies in our intestines help digest fats and carbohydrates, facilitating the absorption of nutrients in cells. Our intestinal microbes also ferment food that we consume. The fermentation process produces chemicals that speeds up our metabolic processes. As a result, the microbes in our gut helps us keep in shape by increasing our metabolism.
  3. Detoxifies us: Microbes living in us are also capable of digesting toxins that we accidently ingest into less harmful substances. Therefore, preventing us from being poisoned. For example, the microbe Lacrobacillus probiotics found in food help the human body detoxify heavy toxic metals such as aluminum.

Good microbes that keep us healthy

Where do we get microbes that live in our body? Most of our microbes that inhabit our intestines comes from the food we ingest. Our skin and lung microbes come from the air we are exposed to. Recently, researchers discovered that newborn infants get their microbes from their mother’s breast milk and vagina. Researchers found that the method of delivery may have an effect on the diversity of intestinal microbes in newborns. They discovered that infants born vaginally and infants born by caesarean section have different intestinal microbe composition. This indicates that we start to develop our microbe colonies from the day we were born.

Microbes inside our body

From Sugar to Morphine

Morphine is an opiate (derived from opium) painkiller, one commonly prescribed for chronic (long-term) and acute (short-term) pain. The current process for making morphine is very extensive and requires farming opium poppies for their poppy straws, from which the morphine is isolated. Morphine is on the Model List of Essential Medicines, formed by the World Health Organization (WHO), which comprise the most important medications for a basic health-care system. Morphine is also used to make other opioids such as oxycodone and methadone.

Image of the opium poppy. Via Wikimedia Commons

Image of the opium poppy. Via Wikimedia Commons

Escherichia coli (E. coli) is a bacterium commonly known for causing food poisoning. Although some E. coli are beneficial and live in our gut, there are many that can cause infections. As the energy-producing processes of these bacteria were similar to the processes involved in making opiates such as morphine, a team of researchers wondered if they could use E. coli to convert sugar into painkillers.

The study, which was published in Nature Communications, was centered around using E. coli to create a precursor to morphine known as thebaine. A precursor is a substance that is used to produce the next substance; thus, thebaine is used to produce morphine. The researchers accomplished this by using four engineered strains of E. coli.

Image of E. coli. Via Wikimedia Commons.

Image of E. coli. Via Wikimedia Commons.

This research has significant implications because of the time it currently takes to produce painkillers. Many poor countries have limited access to opioids, or no access at all. Other production techniques, such as using genetically engineered yeast to produce painkillers, are not efficient enough to challenge tradition opium poppy farming. By using E. coli, the researchers were able to produce 300 times more of the opioid precursor. Another benefit in using bacteria for the production of opioids is that it would only require simple sugars, such as glucose. The yeast production method also faced ethical issues, as anyone that had access to the yeast strain could produce the opioids, leading to the production of street drugs. The E. coli method does not face this problem as the bacteria are difficult to manage and require expert handling.

Image showing heroin, a common street drug. Via Wikimedia Commons.

Image showing heroin, a common street drug. Via Wikimedia Commons.

Once the technique has improved further to increase its efficiency and passed all pharmaceutical regulations, the manufacturing of opioids from bacteria could help solve the deficit in painkillers that the world faces.

Kush Khanna

Krokodil: A Drug That Literally Turns You Into A Zombie

Warning: there are graphic images in this blog post.

While drug usage and addiction has been a problem throughout history, drugs have not always been the easiest vice to access. This may be due to barriers such as strict drug control rules in countries or simply, the drug in question being extremely pricey. However, what if a drug that is relatively unknown and yet sold at lower prices relative to its counterparts suddenly emerged?

Enter Desomorphine, or more commonly known by it’s street name, Krokodil. Despite being around in Siberia since 2002, it only recently hit the shores of North America. This little known drug is an alternative to heroin and is derived from morphineSimilar to other hard drugs, it is highly addictive.

Gangrene

Gangrene is just one of the few deadly effects of Krokodil usage.

However, what sets Krokodil apart from the pack is its deadly effects. Research has found that injecting Krokodil could cause severe damage to the body. Liver and kidney damage as well as rotting gums and bone infection are common problems; with necrosis, gangrene and blood poisoning as icing on top of this very deadly cake. Continued injection of Krokodil could cause a problematic build-up of gangrene  which would lead to amputations or even, a loss of life. 

Of course, Krokodil would not be such a  big problem if it was harder to access compared to other hard drugs. Unfortunately, this is just the opposite. In a recent news site, it was reported that Krokodil prices in Russia go for as low as 5 euros; this, being 10 times lower than Heroin which gooes for aroound 50 euros. With this absurdly low price barriers, many drug addicts who are strapped for cash and need a quick and cheap high could turn to Krokodil as an alternative. It is important that the deadly effects of Krokodil be highlighted so that action can be taken to combat the usage of this drug and people can be educated about the severe risks that lie in its usage. 

A drug that gives a high similar to heroin but at a fraction of the price? To an addict this may sound like the ideal alternative but many fail to read the fine print; another price to pay for their high is to be slowly turned into a rotting pile of flesh. A zombie.

The video below helps to give insight on the lives of Krokodil addicts.

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Credit: Time on Youtube

Vanadium. Can it replace insulin and cure diabetes?

Vanadium (V) is a chemical element located in the d-block of the periodic table as part of the transition metals. Vanadium exists as a trace metal in both plants and animals and vastly abundant in Earth’s crust and sea water. This Vanadium has become a big issue over past decades as it could possibly replace the usage of insulin as to treat diabetes mellitus (DM).

Image from Wikipedia.

Image from Wikipedia.

Vanadium mimics insulin. Just like insulin, vanadium lowers glucose level in the blood, improves insulin sensitivity and normalizes liver enzyme activities. However, insulin has couple drawbacks as it must be injected as it is a protein and gets digested in our stomachs before our bodies absorb. Unlike insulin, vanadium can be orally taken as metals without getting digested in the stomach and can be readily absorbed through the guts solving the discomfort of injecting insulin.

 

Moreover, vanadium is safe. According to an article, doses of up to 400 mg per day is even in the safe level where the recommended dose for a patient is 100-150 per day. In general, the toxicity of vanadium is very low. A study denote most of the toxic effects of vanadium result from local irritation of the eyes and upper respiratory tract rather than systemic toxicity.

 

Not only vanadium is used for diabetes treatment, but also for cancer and and bone formations. Although clinical data and researches are lacking, laboratory studies on human cells demonstrate that vanadium could help promote apoptosis and suppress the growth of cancerous tumours. Furthermore, tests on animals and human cells suggest that vanadium may promote osteogenesis.

 

We do not completely know the virtue of vanadium due to lacking experiments and testing. But many papers and researches have proved vanadium is safe and efficient which could potentially replace the use of insulin in the future.

 

This is a youtube video of a pharmacist explaining why vanadium is important.YouTube Preview Image

 

Sung Eun Kim

Benefits of Breastfeeding: Breast Milk Contains Sugars that Contribute to Healthy Infant Growth

Were you aware that the thousands of bacteria residing in our gastrointestinal tract (gut) and their interactions with the dietary foods we consume actually have a great impact on human health?

Two 25-milliliter samples of human breast milk. The lefthand sample is first milk produced and the righthand sample is milk produced later during the same pumping.

Two 25-milliliter samples of human breast milk. Source: Wikipedia Commons

Inspired by this discovery, a team of researchers led by Jeffrey Gordon from the Washington University School of Medicine in St.Louis found that the interactions of gut bacteria with the sugars in breast milk promote healthy infant growth.

Why is healthy infant growth a topic of discussion?
Every year, childhood malnutrition causes over 3 million deaths, leads to stunted growth and is associated with impaired cognitive ability.

Branched Oligosaccharide Structure

Branched Oligosaccharide Structure.                  Source: Wikipedia Commons

In Malawai, Africa, almost 50% of children under the age of 5 showed stunted growth. The researchers collected samples of human breast milk from those mothers with healthy babies or stunted babies. They discovered that the amount of oligosaccharides (sugar) in the breast milk containing sialic acid, an essential nutrient for brain development and cognition, were much greater in the mothers with healthy, relative to stunted growth babies.

This finding suggests that the sugars in the breast milk contribute to healthy infant growth. To analyze whether this was the case, the researchers created animal models, ensuring that both the bacteria in the gut and the diet could be manipulated. Gordon and his team began by isolating bacterial strains from fecal matter of the undernourished babies and inserted it into mice or piglets. Then, the researchers fed the mice or the piglets a typical Malawian diet, consisting of legumes, corn, vegetables, and fruit, a diet itself which is insufficient for healthy growth.

Whey

Whey, a by-product of cheesemaking. Source: Wikipedia Commons

With the mice and the piglets mimicking the undernourished Malawian infants, Gordon and his team then began testing effects of the sialic acid-containing sugars. They used cow milk as an alternative because of the difficulty to purify large amounts of sugars from human breast milk. They were able to obtain sialic acid-containing sugars from whey, a by-product of cheesemaking, and fed it to the animals. The mice and piglets showed significant improvements in growth, in both muscle mass and in bone volume. The mice and piglets also showed improved brain development and metabolic activities in the liver.

Because the bacteria in the gut was allowed to be manipulated, the researchers were able to pinpoint which bacterial strains were affected by sialic acid-containing sugars and how the different strains interacted with one another. They found that one strain of bacteria fed on the sialic acid-containing sugars and another strain fed on the digested products of sialic acid-containing sugars. This revealed a possible food web within the bacterial gut community.These two strains of bacteria alone were not sufficient enough to explain healthy growth in the mice and piglets, signifying that more complex interactions among different bacteria in the gut were necessary for growth.

The results of their study were recently published in Cell and serves as the foundation for future studies on the benefits of the components of breast milk on healthy infant growth and its interaction with gut bacteria.

 

Posted on February 22, 2016 By Jenny U

Nanotechnology: The Cure for Cancer?

What is Cancer (disease)?

Cancer is a very well known disease plaguing human society for the last century. It is characterized by abnormally rapid cell division often accompanied by tumor growth; the type of tumor mainly being malignant to be more precise. It is

Cigarette, Source: Wikimedia Commons

Cigarette, Source: Wikimedia Commons

known to sprout from sources known as carcinogen (such as ultraviolet radiation and smoking cigarettes) as well as genetic dispositions.

 

Current Treatment

Current treatments are quite limited and do not have an 100% success rate since there are so many mutations and varieties in cancer. Treatments such as chemotherapy often have lasting side effects. Additionally, there is a likelihood that cancerous cells are not completely eradicated and may resurface in years to come.

The following is the background behind chemotherapy and the effects it has on the human body provided by Dnews on YouTube.

What the Future Holds?

What scientists are trying to obtain is a sustainable as well as safe method of treating cancer. Scientists are looking into stem cell research, protein inhibition (protein Kinase inhibition) and artificial intelligence as potential methods. But, perhaps one of the most promising of the potential treatments is nanotechnology.

*Credit to Inquisitr.com for providing these different potential treatment methods.*

Nanotechnology and Cancer Treatment

Nanoparticles are as small as cell organelles with great flexibility when it

Mesoporous silica, Source: Wikimedia Commons

Mesoporous silica, Source: Wikimedia Commons

comes to shape, size and composition. Research by scientists have found a mesoporous silicon nano particle that is able to encase azobenzene molecules within its membrane pores as well as carry different sized substrates inside the particle itself.  It is important to note the fact that azobenzene switches its orientation/ configuration (cis/trans) when exposed to light and its size changes when going from cis to trans. This  means that the permeability of the silica nanoparticle can be regulated by the azobenzene molecular configuration.  Small pellets of anti-cancer drugs can be inserted into the silica nanoparticles and injected into the human body. With controlled light exposure and intensity, the size of azobenzene changes releasing the substrate into the cancer cell targeting either just the cancerous components or the entire cell.  The following video uploaded by FGRGAnimation made by Frank Gu from the University of Waterloo outlines the process of osmosis and cell substrate concentration in regulating and controlling transport in a nanoparticle.

This process will be safe due to the high control levels in the real (in vivo) environment. We are able to selectively insert the amount of substrate into the nanoparticles to control concentration and light intensity. Thus, the correct dosage is always applied. Interestingly, the treatment  process can be both selective or general meaning scientists are able to use substrate specifically targeting cancerous components in a cell or the entire cancer cell.

Although the focus was on nanoparticles and nanotechnology, any of the the aforementioned 4 methods can be an important breakthrough in cancer treatments if proven experimentally successful.

Posted by Ming Lun (Allan) Zhu

 

Martian Life a Possibility

Last September, NASA scientists, using image spectroscopy on the Mars Reconnaissance Orbiter, had published new evidence in support of the hypothesis that present day water flows on the surface of Mars. It is often said that where there is water, there is usually life; but what kind of life can exist in such an extreme environment?

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A recently published study by a group of European Researchers has shown that certain fungi and lichens on Earth have the ability to survive in conditions similar to Mars for an extended period of time. The study is ongoing and will involve several more Fungi and Lichen species as part of the Lichens and Fungi Experiment (LIFE).

"Lichen" by Brittany. From Flickr. Licensed under CC 2.0.

“Lichen” by Brittany. From Flickr. Licensed under CC 2.0.

The two types of fungi collected come from the cryptoendolithic family: the Cryomyces antarcticus and Cryomyces minteri. The organisms were taken from within cracks of rocks found in the McMurdo Dry Valleys in Antarctica. Strong winds, cold temperatures and no precipitation characterize this region. The samples were placed in cells and attached to the ISS via the EXPOSE-E platform.

EXPOSE-E Platform Installation. From Astronomy Magazine.

EXPOSE-E Platform Installation. From Astronomy Magazine.

For a year and a half, half the fungi were subject to Mars-like conditions while the others were exposed to regular conditions in space. The results show that not only did 60% of cells remain unharmed but many were able to resume and even increase cellular activity.

If these lifeforms can survive on the surface of Mars, it may be possible that Martian life already exists. The challenge now for NASA, the European Space Agency and other scientists is to send humans to Mars without contaminating the region.

Do you think that within the century we will discover the first examples of extraterrestrial life?

-Johnny Lazazzera

What Is Skin Cancer? and How Can We Prevent It?

Summer is the best season for sun activities, but the sun exposure is not without a price. As many people already know, skin cancer is mostly developed through sun exposure. Obviously, the best way to prevent skin cancer is to avoid the sun, but we all are eagerly anticipating fun in summer; we want to have pool parties and BBQs in hot and sunny weather. Now, I want to suggest to you the most effective and realistic way to prevent skin cancer when you have fun outside.

The Types of Skin Cancer

First, it is important to know the types of skin cancer because one is more dreadful than other. There are two main types of skin cancer: Squamous Cell Carcinoma and  Melanoma. The first one is less harmful; it only stays in one location of a body. Melanoma, on the other hand, spreads to other areas of a body, especially to other organs. Melanoma is usually darkish, but it can be any colour. In men, it is usually found on the back, whereas in women, it is often found on the leg. 

skin-cancer-chart-700x437

Credit: simpleorganiclife.org

The Risk Factors of Skin Cancer

Patients with light-complexioned skin, naturally blond or red hair, a family history of skin cancer, or many moles have a higher risk of getting skin cancer.

The Ways to Prevent Skin Cancer

It is important to cover up your skin as much as you can. For instance, you should try to wear a hat to protect your face; you should wear sleeves and pants.

You should monthly check your skin, making sure that there is nothing suspicious on your skin. If you think you have skin cancer, you should not hesitate to see doctors.

It is essential to use sunscreen; it is by far the best way to prevent skin cancer. However, you should make sure you use the sunscreen with SPF 30 or higher, with stated “UVA and UVB” protection on the bottle, and the ingredients “titanium” and/or “zinc oxide.”

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Having fun in summer is definitely not wrong, but you should be careful. If you still want sun activities, you should make sure you follow the suggestions I made above, and I hope everyone has a great summer in the future and stays healthy.

skincancer_illustrations

Credit: advanceddermatologypc.com

 

Dohoon Kim